摘要
目的 :探讨肺癌发生的分子遗传学机理 ,了解肺癌中K ras基因的突变情况。方法 :采用聚合酶链式反应一单链构象多态性 (PCR SSCP)结合银染技术 ,对 3 2例肺癌组织及其相应的癌旁组织中K ras基因第 12、13、61位密码子的突变情况进行检测。结果 :肺癌组织中K ras基因总突变率为 2 5 % ( 8/3 2 ) ,其中腺癌突变率为 40 % ( 4 /10 ) ,鳞癌突变率为 2 3 % ( 3 /13 ) ,大细胞肺癌突变率为 3 3 % ( 1/3 )。小细胞癌突变率为 0 ( 0 /6) ,相应的癌旁组织突变率为 3 1% ( 1/3 2 )。结论 :K ras基因突变与肺癌发生发展有关。
Objective: To detect the relation of K ras gene the development of lung cancer.Methods: We used PCR SSCP methods and silver staining technique to analyse the mutations of the 12,13,61 codes of K ras gene in 32 cases lung cancer samples.and their normal tissues around these cancer samples.Results: Mutations of K ras gene were 8 cases mutation positive which yielded a total mutation rate of 25%(8/32),Mutation of K ras gene were detected in 4 of 10(40%) adenocarcinomas cell carcinomas,3 of 13(23%) squamous cell carcinomas,1 of 3(33%) big cell lung carcinomas.Conclusion: K ras gene mutation was a common event in lung cancer which plays an important role during lung cancer.
出处
《河南医学研究》
CAS
2000年第2期134-135,共2页
Henan Medical Research
基金
河南省科委资助!项目 ( 981170 83 5 )
关键词
肺癌
基因
K-RAS
PCR-SSCP
lung cancer
K ras gene
polymerase chain reaction single strand conformation polymorphism
