摘要
目的 :研究氧氟沙星联用抗结核药的肝毒性并探讨其机制。方法 :分别测定异烟肼组、利福平组、异烟肼利福平联合用药 (IR)组及其联用氧氟沙星 (IRO)组小鼠的血清谷氨酸氨基转移酶 (ALT)、肝指数、肝匀浆丙二醛(MDA)含量、肝微粒体细胞色素p45 0和线粒线Ca2 + ATP酶活性 ,及肝病理检查 ,结果与对照组以及实验组之间进行比较。结果 :与正常对照组及单用异烟肼、利福平组比较 ,IR组及IRO组的血清ALT、肝细胞匀浆MDA明显增高 (P <0 0 5 ) ;同时发现IR组和IRO组的肝微粒体细胞色素p45 0含量较高和线粒体Ca2 + ATP酶活性较低 ;病理检查IRO和IRC组肝细胞坏死程度稍轻但与IR组比较无明显差异。结论 :异烟肼和利福平联用氧氟沙星肝毒性增加 ,但并不比异烟肼和利福平联合时强。
Objective: To study hepatic toxic injury induced by ofloxacin with Antituberculosis drugs in mice. Methods: The level of serum glutamic pyruvic transaminase(ALT),liver index,content of malondialdehyde (MDA) of liver homogenate,activity of hepatic microsome enzyme and mitochondrial membrane Ca 2+ ATP enzyme,and degree of liver cell injury were investigated in mice which are in control group and the experimental groups of isoniazid (Ⅰ),rifampicin (R),combining isoniazid with rifampicin(IR),combining ofloxacin with isoniazid and rifampicin(IRO). Results: The groups of IR,IRO could markedly increase the level of ALT,and content of MDA of liver homogenate ( P <0 05) and decrease the activity of mitochondrial membrane Ca 2+ ATP enzyme ( P >0 05),but IRC doesn′t induced these alterations.IR,IRO could increase the degree of liver injury,but there was no difference among the groups of IR,IRO.Conclusion: Combination ofloxacin with isoniazid and rifampicin in mice increase hepatic toxic injury,but the level of injury was little lower than combination isoniazid with rifampicin.
出处
《河南医学研究》
CAS
2000年第2期125-127,共3页
Henan Medical Research
关键词
氧氟沙星
肝毒性
膜脂质过氧化
结核病
ofloxacin
hepatic toxic injury
lipid peroxidation
mitochondrial membrane Ca 2+ ATP enzyme
