摘要
[目的]比较两种培养基下铜绿微囊藻、蛋白核小球藻和四尾栅藻的生长情况。[方法]选用两种培养基(M11和水生四号)对藻铜绿微囊藻、蛋白核小球藻和四尾栅藻进行培养,观察并计算培养期间的藻密度和比增长率。[结果]在温度26℃、光强3 000 lux、光暗比12 h∶12 h条件下,使用M11培养基培养3种藻所得最大藻细胞浓度均大于水生四号培养基的。铜绿微囊藻的μmax和μave在水生四号培养基中更大,蛋白核小球藻和四尾栅藻的μmax和μave均在M11培养基更大。[结论]总体来讲,M11培养基比水生四号培养基更适合这3种藻的实验室培养。
[Objective] The research aimed to contrast growth situations of the Microcystis aeruginosa,Chlorella pyrenoidosa and Scenedesmus quadricauda under two kinds of media.[Method] These three kinds of algae were cultured in M11 and Shuisheng No.4 media.The concentration and specific growth rate of algae cell were observed and calculated.[Result] Under the condition of 26 ℃,3 000 lux and 12 h∶12 h,their maximum cell concentrations in M11 were all higher than that in Shuisheng No.4 medium.μmax and μave of the M.aeruginosa were bigger in Shuisheng No.4 medium,while μmax and μave of the C.pyrenoidosa and S.quadricauda were bigger in M11 medium.[Conclusion] Overall,M11 medium was more suitable for culturing these three kinds of algae in laboratory than Shuisheng No.4 medium.
出处
《安徽农业科学》
CAS
2012年第33期16333-16335,共3页
Journal of Anhui Agricultural Sciences
基金
北京市城市雨水系统与水环境生态技术学术创新团队项目(PHR201106124)
