摘要
目的 观察格林 巴利综合征 (GBS)患者及其亚组的HLA基因分布与正常对照的差异 ,以寻找GBS的遗传易感性及其与GBS自身免疫因素的关系。方法 采用参照Olerup等的文献合成的序列特异性寡核苷酸引物 ,用PCR SSP方法对 4 7例GBS患者、7例CJ肠炎和 50例正常对照进行HLA分型。结果 在整个GBS组、CJ组、有CJ近期感染的GBS组、IgG型和IgM型GM1抗体阳性的GBS组 ,HLA DQA、 DQB和 DRB基因的分布与正常对照的差异无显著性 (P >0 .0 5)。DQA1 0 30 2在有CJ近期感染的GBS组有增高趋势 (x2 =2 .936 ,P=0 .0 87) ,RR =3.587;DQA1 0 30 1在IgG型GM1抗体阳性的GBS患者有增高的趋势 (x2 =3.6 59,P =0 .0 51) ,RR =2 .991。结论 目前没有发现GBS及其亚组中HLA DQA1、 DQB1和 DRB基因的频率与正常对照组存在差异 ,但是个别基因的频率表现出增高的趋势 ,可能存在与特定的免疫因素之间的关系 ,有待于扩大样本进一步观察。
Objective To stady whether genetic factors may play in concert with autoimmunological factors in the pathogenesis of GBS by determining the difference of HLA alleles between GBS Patients and normal controls. Methods Sequence specific primers of HLA DQA, DQB and DRB alleles were synthesized according to Olerup et al. And HLA was typed by PCR SSP methods in 47 GBS, 7 CJ enteritis patients and 50 normal controls. Results There were no difference of the frequency of HLA DQA, DQB and DRB among GBS group, CJ group, normal controls and GBS subgroups in respect to recent CJ infection,associated with GM1 IgG and GM1 IgM antibodies. But there were increasing tendency of DQA1*0301 in the subgroup with IgG GM1 antibodies ( x 2 =3.659, P =0.051, RR =2.991) and DQA1*0302 in the subgroup with recent CJ infection ( x 2 =2.936, P =0.087, RR =3.587). Conclusions No difference was found among GBS, CJ enteritis patients and normal controls. But the increasing tendency of the two alleles suggests there may be some relation between genetic factors and immunological factors, a definite conclusion waits for further investigations.
出处
《中国神经免疫学和神经病学杂志》
CAS
2000年第2期87-93,共7页
Chinese Journal of Neuroimmunology and Neurology
基金
国家自然科学基金!资助项目 (39470 2 5 9)
关键词
格林-巴利综合征
人类白细胞抗原
基因分型
PCR
Guillain Barre syndrome
human leukocyte antigens
polymerase chain reaction
genotyping
campylobacter jejuni
