摘要
[目的]优化酸枣根鞣质的提取工艺,建立稳定的酸枣根中鞣质的含量测定方法。[方法]用干酪素法测定总鞣质的含量,并以提取物中鞣质含量为评价指标,以丙酮浓度、提取时间、料液比及提取次数为考察因素,采用正交设计法筛选酸枣根的提取工艺条件。[结果]提取次数为主要影响因素;最佳提取条件为:超声提取的丙酮浓度为70%,料液比为1∶20(W/V,g/ml,下同),提取时间为30 min,提取3次;在此条件下,酸枣根中鞣质的含量为6.82%。[结论]所优选的鞣质提取工艺合理,操作简便可行。
[Objective] To optimize the extraction technology of tannins from Ziziphus jujuba roots,and to establish stable determination method of tannins content in Z.jujuba roots.[Method] Content of total tannins was detected by casein method.With tannins content as the evaluation index,the extraction time,solid-liquid ratio,extraction times,and acetone concentration were taken as the investigation factors.Extraction technology conditions were optimized by orthogonal design.[Result] Results showed that extraction times was the major influencing factor.The optimal extraction condition was as follows： 70% acetone concentration,1∶20（W/V,g/ml）solid-liquid ratio,30 min extraction time and 3 extraction times.Under this condition,the tannins content in Z.jujuba roots reached 6.82%.[Conclusion] The optimized extraction technology was reasonable,simple and feasible.
出处
《安徽农业科学》
CAS
2012年第33期16107-16108,16418,共3页
Journal of Anhui Agricultural Sciences
关键词
酸枣根鞣质
干酪素法
含量测定
正交设计
提取工艺
Tannins in Z.jujuba roots
Casein method
Content determination
Orthogonal design
Extraction technology
