摘要
目的 :研究雄激素受体 (AR)基因突变与前列腺癌 (PC)发生、发展的关系。方法 :采用自行设计的 3对引物 ,检测了 2 5例PC石蜡切片组织 ,对其AR的N端转录激活区进行了银染聚合酶链式反应 单链构象多态性分析 (PCR SSCP)和异常SSCP片段的DNA测序。结果 :发现一患者的A片段有SSCP异常 ,经测序证实A外显子存在一错义突变 (C96 6 →A ,Ser2 96Arg)。结论 :这是一新发现的突变 ,这一突变位于转录激活区内 ,推测可改变AR的转导活性 ,从而影响AR的功能。
Objective:To study the relationship between the mutation of androgen receptor(AR) gene and the development of human prostate cancer(hPC).Methods:With the 3 pairs of primers designed by ourselves,we detected the transcriptive dormain of N terminal of AR gene in 25 paraffin embedded slides of hPC by polymerase chain reaction single strand conformation polymerphism(PCR SSCP).The abnormal shift band was further detected by direct DNA cycle sequencing.Results:One mobility difference was found by SSCP.From this shift band we identified one point mutation in 296 amino acid (C 966 →A,Ser296Arg).Conclusions:It's an unreported point mutation.This result might change the transcriptive activity of AR.Further studies are needed to evaluate the effects of this mutation on AR function.
出处
《肿瘤防治杂志》
2000年第2期143-145,共3页
China Journal of Cancer Prevention and Treatment
基金
国家自然科学基金!(396 70 30 0 )资助
关键词
前列腺癌
雄激素受体
基因突变
prostate cancer
gene mutation
androgen receptor
