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乌司他丁对脂多糖诱导的巨噬细胞炎症反应的影响 被引量：1

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摘要 目的探讨乌司他丁对LPS诱导的RAW264.7细胞炎症反应的影响。方法采用1.0μg/mL的LPS激活RAW264.7细胞株,与不同浓度组乌司他丁(100～10 000 U/mL)共同孵育,采用ELISA法测定TNF-α和IL-1β的表达;实时荧光定量逆转录聚合酶链反应(RT-PCR)技术分析TNF-αmRNA和IL-1βmRNA表达。结果高浓度乌司他丁(1 000～10 000 U/mL)可抑制LPS诱导的RAW264.7细胞TNF-α和IL-1β表达(P均<0.05),下调LPS诱导的RAW264.7细胞TNF-αmRNA和IL-1βmRNA含量(P均<0.05);低浓度乌司他丁(100 U/mL)对LPS诱导的RAW264.7细胞TNF-α、IL-1β、TNF-αmRNA、IL-1βmRNA表达与LPS单独处理组比较,无显著差异。结论乌司他丁可抑制LPS诱导的巨噬细胞炎症反应;该抑制作用呈浓度依赖性。
作者 黄春燕 赵艳琼 邝晓聪
机构地区 广西医科大学研究生学院 广西医科大学公共卫生学院 广西医科大学基础医学院病理生理学教研室
出处 《山东医药》 CAS 2012年第39期35-36,共2页 Shandong Medical Journal
关键词 乌司他丁 巨噬细胞 炎症因子 脂多糖
分类号 R453 [医药卫生—治疗学]
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参考文献6

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二级参考文献14

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山东医药
2012年 第39期

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