摘要
运用地高辛标记纤维连接蛋白 (FN)特殊位点的探针 ,通过原位杂交方法检测不同损伤时间大鼠皮肤各型FN剪接异型体的表达情况 ,结果发现 :伤后大鼠皮肤各型FN的表达随时间呈增高趋势 ;伤后 30～ 6 0min表达出正常皮肤中没有的EⅢA、EⅢB片段。FN的这一特性为法医学诊断生前、死后伤和伤后存活时间推断提供了新指标。
In situ hybridization with Digoxingenin labeled probes were used to build the relationship between incised cutaneous wounding intervals and the expression of splicing fibronectin variants (total, EⅢA and EⅢB Variants) in rats The results showed that all forms of FN variants increased notably with the wounding time going on EⅢA and EⅢB Variants were expressed abundantly 30 and 60 minutes after injury, while few in normal skin
出处
《法医学杂志》
CAS
CSCD
2000年第2期70-71,74,共3页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目!(No 39770 816 )
关键词
纤维连接蛋白
损伤时间推断
高辛
法医鉴定
fibronectin
estimation of wounding interval
digoxingenin
in situ hybridization
