摘要
目的:探讨肺结核患者外周血CD4+和CD8+记忆性T细胞亚群及白细胞介素17(Interleukin-17,IL-17),IL-27的表达。方法:采用流式细胞术检测肺结核患者组外周血CD4+和CD8+记忆性T细胞,患者组按治疗史分为初治组,复治组;按痰涂片抗酸染色分为痰涂阳性组,痰涂阴性组。初治组8例为凡既往未用过抗结核药物治疗或用药时间少于一个月的新发病例;复治组22例为凡既往应用抗结核药物一个月以上的新发病例、复发病例、初治治疗失败病例等;痰涂阳组9例为痰涂片抗酸染色阳性;痰涂阴组21例为痰涂片抗酸染色阴性。ELISA法检测IL-17,IL-27。结果:①与健康对照组比较:患者组CD4+效应型记忆性T细胞均显著增高(P<0.05),初治组与涂阴组CD8+效应型记忆性T细胞均显著降低(P<0.05),复治组IL-17显著降低(P<0.05),而IL-27显著增高(P<0.05)。②与初治组比较:复治组CD4+中央型记忆性T细胞显著降低(P<0.05),而CD8+效应型记忆性T细胞显著增高(P<0.05),IL-17显著降低(P<0.05),IL-27显著增高(P<0.05)。③与涂阴组比较:涂阳组CD4+中央型记忆性T细胞显著降低(P<0.05),CD8+效应型记忆性T细胞显著增高(P<0.05),CD8+中央型记忆性T细胞显著降低(P<0.05)。④Spearman相关性分析显示:IL-17与IL-27呈正相关(P<0.05),记忆性T细胞亚群与IL-17,IL-27水平无显著相关性(P>0.05)。结论:肺结核患者外周血CD4+效应型记忆性T细胞的表达可望作为TB初步诊断的观察指标,CD4+中央型记忆性T细胞和CD8+效应型记忆性T细胞的表达均可望作为TB患者的临床分组依据,TB外周血IL-17、IL-27水平可望用于判断TB的炎症程度。
Objective：To investigate the expression levels of CD4^＋ and CD8^＋ memory T cell subsets,interleukin-17 and interleukin-27 in patients＇ peripheral blood with pulmonary tuberculosis.Methods：We have detected the expression of CD4^＋ and CD8^＋ memory T cells by flow cytometry in peripheral blood with pulmonary tuberculosis patients.Patients have been grouped first therapy group and repeat therapy group by therapy history,smear positive group and smear negative group by sputum smear acid-fast staining.8 persons of first therapy group were previously unused antituberculosis drug therapy or medication time less than one month of new onset.22 persons of repeat therapy group were the previous application of antituberculosis drugs for more than a month of the new cases,and recurrent cases,primary treament failure cases.9 persons of sputum smear acid-fast staining positive was smear positive group and 21 persons staining negative was smear negative group.We have analyzed the levels of IL-17 and IL-27 by ELISA.Results：①Compared with the healthy：CD4^＋effector memory T cells increased significantly in patients group（P〈0.05）,CD8^＋ effector memory T cells decreased significantly in first therapy group and smear negative group（P〈0.05）,IL-17 decreased significantly in repeat therapy group（P〈0.05）,however,the level of IL-27 increased significantly（P〈0.05）.②Compared with the first therapy group： CD4^＋ central memory T cell decreased significantly in repeat therapy group（P〈0.05）,CD8^＋effector memory T cells increased significantly（P〈0.05）,IL-17 decreased significantly（P〈0.05）,and IL-27 increased significantly（P〈0.05）.③Compared with smear negative group：CD4^＋central memory T cells decreased significantly in smear positive group（P〈0.05）,CD8^＋effector memory T cells increased significantly（P〈0.05）,however,the CD8^＋central memory T cells significantly decreased（P〈0.05）.④Spearmcorrelations：there was positive correlation between IL-17 and IL-27（P〈0.05）.No correlations between memory T cell subgroups and IL-17/IL-27 levels（P〉0.05）.Conclusion：Our results suggested that the expression of CD4^＋ effector memory T cell subgroup in peripheral blood with pulmonary tuberculosis patients may be used as a clinical marker for pulmonary tuberculosis diagnosis.The CD4^＋central memory T cells and CD8^＋ effector memory T cells could be as a basis of clinical stage for pulmonary tuberculosis patients.The level of IL-17 and IL-27 is expected to used to determine the severity of the TB.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第10期930-935,共6页
Chinese Journal of Immunology
基金
贵州省卫生厅2009年度科学技术基金项目(gzwkj2009-1-069)
合肥医学院配套基金项目(D-364)
合肥医学院附属医院中青年启动基金项目(209.001.113.19)
