摘要
目的离子型受体类药物能快速有效缓解抑郁症状,但其作用机制尚不明确。文中探讨N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体及其亚型受体拮抗剂对强迫游泳大鼠不动时间及海马脑源性神经营养因子(brain-derivedneurotrophic factor,BDNF)表达的影响。方法雄性Wistar大鼠40只,采用随机数字表法,分为4组,每组10只。药物干预前1 d大鼠强迫游泳15 min,药物干预当天,分别腹腔注射1 ml容积等渗盐水(S组)、NVP-AAM077(NR2A受体拮抗剂)5mg/kg(NVP组)、氯胺酮(NMDA受体拮抗剂)10 mg/kg(Ket组)、Ro25-6981(NR2B受体拮抗剂)10 mg/kg(Ro组)。30 min后,记录强迫游泳大鼠不动时间,取大鼠海马组织检测BDNF。结果与S组相比,NVP组大鼠强迫游泳不动时间及海马BDNF的表达均无显著变化,而Ket组及Ro组大鼠强迫游泳时间均显著减少且海马BDNF的表达均显著增加(P<0.05)。结论氯胺酮及Ro25-6981均具有抗抑郁作用,其作用机制可能与海马BDNF的上调有关。
Objective The ion receptor drugs relieve the symptons of depression, while the mechanism for this effect is un- clear. The aim of this study is to investigate the effect of the N-Methyl-D-aspartate （NMDA） receptor and its sub-receptor antagonists on the immobility time and expression of the hippocampual brain-derived neurotrophic factor （BDNF） in rats receiving the forced swim- ming test （FST）. Methods Forty male Wistar rats were equally randomized to four groups. After insulted in FST for 15 min on the previous day, the rats were intraperitoneally injected with 1 ml of saline （the S group） , 5 mg/kg of NVP-AAM077 （NR2B receptor an- tagonist） （the NVP group）, 5 mg/kg of ketamine （NMDA receptor antagonist） （the Ket group）, and 10 mg/kg of Ro25-6981 （NR2B receptor antagonist） （the Ro group）, respectively. Thirty minutes later, the immobility time of the rats receiving FST was re- corded, and the hippocampus tissue was harvested for detection of the level of BDNF. Results Compared with the rats in the S group, those in the NVP group exhibited no significant changes either in the immobility time nor in the expression of hippocampuaJ BDNF （ P 〉 0.05 ）, whereas the Ket and Ro groups showed a remarkable decrease in the immobility time and an increase in the expres- sion of hippocampual BDNF （P 〈 O. 05 ）. Conclusion Both Ketarnine and Ro25-6981 have an antidepressant effect, which may be related to the upregulated expression of hippocampual BDNF.
出处
《医学研究生学报》
CAS
北大核心
2012年第10期1012-1014,共3页
Journal of Medical Postgraduates
基金
国家自然科学基金(30872424)
全军"十二五"医药卫生科研基金(CWS11J017)
