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犬牙周膜细胞和骨髓基质细胞相互作用的体外实验研究 被引量：1

Study on the Interaction of Periodontal Ligament Cells and Bone Marrow Stromal Cells in vitro
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摘要 目的:研究牙周膜细胞(PDLCs)和骨髓基质细胞(BMSCs)共培养是否更利于牙周组织再生。方法 :用组织块法培养PDLCs,用全血贴壁法培养BMSCs,检测它们的成骨、成脂诱导分化能力。用Transwell小室共培养这两种细胞,通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)和实时荧光定量PCR(Real-time PCR)检测共培养后这两种细胞的增殖能力及部分基因的表达变化。结果:PDLCs和BMSCs具有多向分化潜能。共培养能促进它们各自增殖,上调Ⅰ型胶原(COLI)、碱性磷酸酶(ALP)、骨涎蛋白(BSP)、骨钙素(OCN)、核心结合因子2(RUNX2)的表达。结论:PDLCs和BMSCs共同运用作为种子细胞可能更有利于牙周组织再生。 Objective： This study was designed to investigate whether the interaction of periodontal ligament cells （PDLCs） and bone marrow stromal cells （BMSCs） is related to better periodontal tissue regeneration. Methods： PDLCs and BMSCs were co-cultured by Transwell chambers. The co-cultured PDLCs and BMSCs were examined by MTT applying 3-（4,5- dimethyhhiazol-2-yl）-2,5-diphenyltetrazolium bromide and Real-time PCR assays. Results： PDLCs and BMSCs manifested multilineage differentiation potential. When co-cultured, proliferation rates of PDLCs and BMSCs raised. Gene expressions of COLI, ALP, BSP, OCN, RUNX2 were upregulated. Conclusion： It may be better to use PDLCs and BMSCs together for periodontal tissue regeneration.
作者 刘曙 刘宏伟
机构地区 同济大学口腔医学院
出处 《口腔颌面外科杂志》 CAS 2012年第5期318-322,共5页 Journal of Oral and Maxillofacial Surgery
基金 国家自然科学基金资助项目(30772418)
关键词 牙周膜细胞 骨髓基质细胞 共培养 犬 periodontal ligament cells bone marrow stromal cells eoeuhure dog
分类号 R780.2 [医药卫生—口腔医学] Q813.11 [生物学—生物工程]
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参考文献13

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同被引文献15

  • 1Taba M Jr, Jin Q, Sugai JV, et al. Current concepts in pe- riodontal bioengineering[J] Orthod Craniofac Res, 2005, 8(4):292-302.
  • 2Zhang P, Men J, Fu Y, et al. Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bone [J]. Cell Tissue Res, 2012, 350(3): 425-437.
  • 3Wang L, Shen H, Zheng W, et al. Characterization of Stem Cells from Alveolar Periodontal Ligament [J]. Tissue Eng Part A, 2011, 17(7-8):1015-1026.
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  • 5Isaka J, Ohazama A, Kobayashi M, et al. Participation of periodontal ligament cells with regeneration of alveolar bone[J]. J Periodontol, 2001, 72(3):314-323.
  • 6Kawaguchi H, Hirachi A, Hasegawa N, et al. Enhance- ment of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells [J]. J Periodontol, 2004, 75(9):1281-1287.
  • 7Hasegawa N, Kawaguchi H, Hirachi A, et al. Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects[J]. J Periodontol, 2006, 77(6):1003- 1007.
  • 8Damek-Poprawa M, Stefanik D, Akintoye SO. Human bone marrow stromal cells display variable anatomic site- dependent response and recovery from irradiation[J]. Arch Oral Biol, 2010, 55(5):358-364.
  • 9Xie H, Liu H. A novel mixed-type stem cell pellet for cementum/periodontal ligament-like complex[J]. J Periodon- tol, 2012, 83(6):805-815.
  • 10Choi EJ, Yim JY, Koo KT, et al. Biology effects of a semiconductor diode laser on human periodontal ligament fibroblasts [J]. J Periodontal Implant Sci, 2010, 40(3):105- 110.

引证文献1

口腔颌面外科杂志
2012年 第5期

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