摘要
目的:建立甲状腺功能低下阳虚模型,为病理研究、药物研发奠定基础。方法:将SD雄性大鼠随机分为正常组、模型组,每天上午定时灌胃薄荷油1.25mL/kg,持续灌胃21d;分别在第14、21天检测血清甲状腺功能、物质能量代谢、肝肾功能等相关指标。结果:薄荷油诱导的阳虚模型组,与正常组相比,在第14天、21天出现三碘甲腺原氨酸(T3)、甲状腺素(T4)显著性降低(P<0.05,P<0.01);葡萄糖(GLU)、甘油三酯(TG)、总胆固醇(TC)、白蛋白(ALB)、钠-钾-腺嘌呤核苷三磷酸酶(Na+-K+-ATPase)与正常组比显著性降低(P<0.05,P<0.01);而其血清中肌酐(Cr)、尿素氮(BUN)、谷丙转氨酶(ALT)、谷草转氨酶(AST)与正常组比较均没有统计学差异。结论:给大鼠灌胃薄荷油1.25mL/kg,在第14-21天可以出现较理想的甲状腺功能低下的阳虚动物模型,且未引起肝肾毒性反应。
Objective： To establish the hypothyroidism with yang-deficiency model for pathological studies and to lay the foundation for drug research and development.Methods： Male SD rats were randomly divided into normal group and model group,fed regularly every morning,peppermint oil 1.25mL/kg,continuous gavage for 21 days;serum thyroid function,material and energy metabolism,liver and kidney function and other related indicators were tested at day 14 and day 21.Results： Yang-deficiency and peppermint oil-induced model group,compared with normal group,at day 14 day and day 21,developed significant reduction of T3,T4;GLU,TG,TC,ALB,Na＋-K＋-ATPase,compared with the normal group,were significantly lower（P0.05,P0.01）;and there was no significant difference of Cr,BUN,ALT,AST between model group and normal group.Conclusion： The rats,fed with peppermint oil 1.25mL/kg,could become satisfied animal model with hypothyroidism and yangdeficiency syndrome,without inducing liver and kidney toxicity.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第11期2907-2910,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家重点基础研究发展计划(973计划)(No.2007CB512605)
国家自然科学基金项目(No.81073068)
高等学校博士学科点专项科研基金资助课题(No.20100013110011)
北京中医药大学科研创新团队项目~~
关键词
薄荷油
阳虚模型
甲状腺功能低下
肝肾毒性
Peppermint oil
Yang-deficiency model
Hypothyroidism
Liver and kidney toxicity
