摘要
目的探讨在哮喘大鼠气道平滑肌细胞(ASMC)中ERK信号通路对Smad6/7蛋白的调控。方法复制哮喘大鼠模型,原代培养ASMC,实验设未干预组(A组)、细胞外调节激酶(ERK)阻断剂(U0126)组(B组)、血小板源性生长因子(PDGF)组(C组)、PDGF+ERK阻断剂组(D组)。免疫组化法测定Smad6/7蛋白的表达,反转录-聚合酶链反应(RT-PCR)法测定Smad6/7 mRNA表达。结果哮喘大鼠ASMC中Smad 6/7蛋白B组显著高于A组(P<0.01),C组显著低于A组(P<0.01),D组与A组比较无统计学差异(P>0.05);各组哮喘大鼠ASMC中Smad6/7 mRNA表达无统计学差异。结论哮喘大鼠ASMC中ERK信号通路调控Smad6/7的作用发生在蛋白水平,不发生于基因水平。
Objective To explore the effect of ERK signal pathway on Smad 6/7 in ariway smooth muscle cells（ASMC） of asthmatic rats. Methods Asthmatic rats were copied. ASMC were cultured from asthmatic rats which were divided into four groups：control group （group A） , external signal regulated kinase （ERK） blocking agent （U0126） group（ group B） , platelet- derived growth factor（PDGF） group（group C） , PDGF plus ERK blocking agent group （group D）. The protein expressions of Smad6/7 were detected by immunohisto- chemistry technique, and the mRNA expressions of Smad6/7 were detected by RT - PCR. Results The expression of Smad6/7 protein in ASMC of group B was significantly higher than group A （P 〈 0.01 ） , and group C were significantly lower than group A （ P 〈 0.01 ）. There were no significant difference between group D and group A（ P 〉 0.05 ）. There were no difference in the mRNA expressions of Smad6/7 a- mong all these groups（P 〉 0.05）. Conclusion The regulatory role of ERK signal pathway on Smad6/7 in ASMC of asthmatic rats was on the level of protein, but not on the level of gene.
出处
《医学研究杂志》
2012年第10期42-46,共5页
Journal of Medical Research
基金
国家自然科学基金资助项目(30571981
30271383)
