摘要
目的 :比较研究绿茶对铝钾矾、环磷酰胺 (Cyclophosphamide,简称 CP)诱发遗传物质损伤的修复作用。方法 :小白鼠骨髓细胞试验微核方法和小白鼠骨髓细胞染色体畸变试验方法。即以绿茶为阻断剂、以铝钾矾、环磷酰胺为诱变剂 ,测定小白鼠骨髓嗜多染红细胞 (PCE)的微核率和染色体畸变率。结果 :含 10 %绿茶的饲料使铝钾矾诱发的嗜多染红细胞微核率从 8.17± 1.6 0‰降至 3.6 7± 0 .82‰ (P <0 .0 0 1) ,染色体畸变率从 14.0 0± 2 .5 3降至 8.17± 1.47 (P <0 .0 0 1) ;含 10 %绿茶的饲料使环磷酰胺诱发的 PCE微核率从 32 .5 0± 3.5 1‰降至 19.33± 2 .5 8‰ (P<0 .0 0 1) ,染色体畸变率从 2 6 .83± 3.82降至 19.17± 2 .93 (P<0 .0 1)。结论 :表明绿茶对铝钾矾诱发微核、畸变的抑制率分别大于 CP诱发微核、畸变的抑制率 (P<0 .0 0 1) ,即铝钾矾诱发遗传物质损伤的抑制率大于环磷酰胺诱发遗传物质损伤的抑制率。
Objective:To study comparatively green tea repairing effects on damaged genetic material by Aluminum Potassium Sulfate or CP.Methods: The micronucleus assay in mice marrow PCE and chromosome aberration assay in mice marrow cells have been used. Results: The feed containing 10% green tea made the rate of micronucleus decline from 8.17±1.60‰ to 3.67±0.82‰(P <0.001)and the rate of chromosome aberration decline from 14.00±2.53% to 8.17±1.47%(P <0.001)by Aluminum Potassium Sulfate. The feed containing 10% green tea made the rate of micronucleus decline from 32.50±3.51‰ to 19.33±2.58‰(P <0.001) and the rate of micronucleus decline 26.83±3.82% to 19.17±2.93%(P <0.01)by CP. Conclusion: The inhibitory rates of green tea to micronucleus rates and aberration rates by Aluminum Potassium Sulfate were higher than those by CP ,i.e.the inhibitory rate of green tea to genetic material damaged by Aluminum Potassium Sulfate was bigger than that by CP(P <0.001). [FK(WB80011。5]
作者
钱晓薇
QIAN Xiao wei(The Department of Biology, Wenzhou Teachers College, Wenzhou 325003)
出处
《温州医学院学报》
CAS
2000年第1期33-34,共2页
Journal of Wenzhou Medical College
关键词
绿茶
铝钾矾
环磷酰胺
染色体突变
green tea
aluminum potassium
sulfate
cyclophosphamide
