摘要
通过对猪增生性肠炎阳性病例肠粘膜进行处理、过滤、分离出胞内劳森氏菌,分别运用抗酸染色、Giemsa染色、银染和PCR检测,并设增生性肠炎弱毒疫苗为阳性对照。结果表明,在显微镜下都能观察到这三种不同染色方法染色的胞内劳森氏菌,且PCR检测均为阳性,与弱毒疫苗阳性对照结果一致。结论:成功分离并鉴定出劳森氏菌,为进一步研究其培养特性和病原学研究奠定了基础。
In this paper,L.intracellularis was isolated from processing the intestinal mucosa of porcine proliferative enteritis and filtering material.Acid-fast staining,Giemsa staining,silver staining and PCR were used to detect L.intracellularis and proliferative enteritis attenuated vaccine was detected as a positive control.The results show that L.intracellularis using these different staining methods can be observed under the microscope and PCR identified as a positive control.The conclusion is that we successfully isolated and identified L.intracellularis,which lays a foundation for further study of its cultural characteristics and etiological research.
出处
《龙岩学院学报》
2012年第5期55-60,共6页
Journal of Longyan University
基金
福建省科技厅区域重大项目(2009N3013)
福建省科技厅青年科技人才创新项目(2010J05077)
关键词
胞内劳森氏菌
分离
检测
Porcine Lawsonia intracellularis
isolation
detection
