基于IL-13、NF-κB p65变化探讨小青龙汤干预咳嗽变异性哮喘临床研究被引量：17

The Clinical Intervention Effect of Minor Decoction of Green Dragon Treating with Cough Variant Asthma Based on the Changes of IL-13 and NF-κB p65

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摘要目的:基于治疗前后IL-13、NF-κB p65以及中医证候变化,主观、客观指标合参,客观评价小青龙汤干预咳嗽变异性哮喘的临床疗效。方法:60例符合诊断标准、纳入标准的咳嗽变异性哮喘患者予以小青龙汤颗粒剂干预4周。详细记录治疗前、后中医证候变化;应用免疫组织化学及Real-time PCR技术检测血清IL-13、NF-κB p65变化。结果:小青龙汤治疗咳嗽变异性哮喘有效率为76.66%,治疗前IL-13蛋白及其mRNA表达呈下降趋势,NF-κB p65蛋白及其mRNA表达呈上升趋势;治疗后IL-13蛋白及其mRNA表达呈上升趋势,NF-κB p65蛋白及其mRNA表达呈下降趋势,与治疗前比较,差异有统计学意义(P<0.05)。结论:小青龙汤可能通过调控IL-13、NF-κB p65表达,具有明确缓解咳嗽变异性哮喘的效用。 Objective：The mechanisms of minor decoction of green dragon interfering with cough variant asthma were discussed by observing the TCM syndrome and the changes of IL-13 and NF-κB p65.Methods：Sixty cough variant asthma patients were administrated by minor decoction granules of green dragon for 4 weeks.The changes of clinical manifestations were documented before and after treatment.The expression of IL-13 and NF-κB p65 were assayed by IHC and Real-time PCR.Results：The total effect rate of minor decoction of green dragon in treating cough variant asthma is 76.66%.Before treatment,the expression of IL-13 mRNA and protein is on the decline.However,the expression of NF-κB p65 mRNA and protein is on the increment.After treatment,the expression of IL-13 mRNA and protein is on the increment.But,the expression of NF-κB p65 mRNA and protein is on the decline.There is a significant difference in the expression of IL-13 and NF-κBp65 before and after treatment（P0.05）.Conclusion：Minor decoction of green dragon exerts an exact effect on alleviating cough variant asthmaby regulating the expression of IL-13 and NF-κB p65.

作者谢嘉嘉梁建华彭俊杰曾小玲林国彬

机构地区深圳市宝安区中医院

出处《中医学报》 CAS 2012年第11期1398-1400,共3页 Acta Chinese Medicine

关键词 IL-13 NF-ΚBP65 小青龙汤颗粒剂咳嗽变异性哮喘临床研究 interleukin-13 nuclear factor-kappa B p65 minor decoction granules of green dragon cough variant asthma clinical study

分类号 R256.12 [医药卫生—中医内科学]

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