摘要
为了探讨几种天然产物对痛风性关节炎模型大鼠的影响,通过对wistar大鼠踝关节注射尿酸钠结晶,制作痛风性关节炎模型,并预先对实验大鼠灌胃给药褐藻糖胶、柠檬酸钾和东哥阿里提取物,通过检测踝关节肿胀度、步态积分及血清样本中炎症因子变化,研究其对痛风性关节炎的预防和缓解作用。结果发现,三种活性物质均可显著降低痛风性关节炎实验大鼠造模24 h踝关节肿胀度;东哥阿里提取物对实验大鼠步态积分有降低作用;褐藻糖胶与东哥阿里提取物对髓过氧化物酶有显著降低作用;东哥阿里提取物可以显著降低炎症因子IL-1β、IL-8、NO和5-HT的血清浓度;因而推测,三种活性物质均对尿酸钠引起的痛风性关节炎有预防和缓解作用,其中东哥阿里提取物在减轻关节肿胀、缓解痛感及抑制炎症发展方面有显著作用。
Gouty arthritis is one of the most painful symptoms of gout patients. This research aimed to investigate the effect of several natural extracts on acute gouty arthritis in rats induced by monosodium urate （MSU） crystals. The animal model of acute gouty arthritis on wistar rats was made through injecting MSU in ankle joints. The rats were administrated orally with Fucoidan, Potassium Citrate and Tongkat All extracts. And the joint swelling, gait score and several inflammatory factors in serum were measured to discuss the prevention and relief effects in the inflammation-related limb swelling, paln,dysfunction of the natural extracts on acute gouty arthritis and investigated the mechanism preliminarily. As a result, the three kinds of natural extracts could significandy reduce the joint swelling after induction MSU in rats 24 h later. This showed that the three could inhibit the symptoms inflammation-related limb joint swelling. Tongkat Ali extracts could decrease the gait score. It means that the limb inflammation, pain and dysfunction had been reduced on the rats. Anti-inflammatory mechanisms of the three active substances were explored, by further testing of experimental rat serum biochemical indices. Fucoida and Tongkat Ali extracts could significantly reduce the level of Myeloperoxidase. It indicated that both the mitigation of the inflammatory response may be related to the activation of neutrophils. And Tongkat Ali extracts could significantly decrease the IL-1β,IL-8 ,NO and 5-HT of model rats. It showed that Tongkat Ali extracts had a certain inhibitory effect on the occurrence, development and other aspects of inflammation, while the symptoms of limb dysfunction was reduced by inhibiting the pain related inflammatory factor such as 5-HT. In conclusion, the three kinds of natural extracts can relieve the attack of acute gouty arthritis induced MSU in rats. And the Tongkat Ali extracts play an important role in reducing the joint swelling, relieving the pain and the inhibition of inflammation.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第10期1362-1366,1416,共6页
Natural Product Research and Development
