摘要
线粒体是真核生物中重要的细胞器,其包含的全部蛋白质称为线粒体蛋白质组。人类线粒体大约包含1 500多种蛋白质,由核基因和线粒体基因共同编码。线粒体是细胞能量合成和物质代谢的中心,其功能障碍将直接或间接引起许多疾病。目前线粒体蛋白质组学正是系统性地研究线粒体在生理、病理过程中的功能变化以及研究疾病发生机制的重要方法。将线粒体蛋白质组的研究方法、研究进展、线粒体蛋白质组的性质及其在相关疾病研究中的作用进行综述,并对线粒体蛋白质组学在疾病发生机制和诊断治疗中的发展前景进行展望。
Mitochondria are important organelles in eukaryotes, entire proteins in the mitochondria are called mitochondrial proteome. Recently human mitochondrial proteome was identified that consisted of 1500 proteins which were encoded by nuclear and mitochondrial DNA. Mitochondria are central to cellular energetic metabolism and material metabolism, while mitochondrial dysfunction will directly or indirectly leads to large numbers of diseases. However the mitochondrial proteome systematically analyses of the organelle in physiology and pathogenesis, also discovers the genetic basis of diseases. In the review, we introduce the methods and recent progress in the field of mitochondrial proteome; then we elaborate properties of the mitochondrial proteome and explore future prospects and challenges for using the proteome for systems analysis of the mitochondria.
出处
《生命科学》
CSCD
2012年第10期1082-1088,共7页
Chinese Bulletin of Life Sciences
基金
卫生部科学研究基金-浙江省医药卫生重大科技计划(WKJ2011-2-011)
温州市科技局重大项目(H20100077)
浙江省自然科学基金项目(Y2080812)
关键词
线粒体
蛋白质组
呼吸链
质谱技术
mitochondria
proteome
respiratory chain
mass spectra
