摘要
以葡萄糖为碳源合成荧光碳点;以巯基琥珀酸为稳定剂,合成CdTe量子点。通过紫外吸收光谱和荧光发射光谱对二者的荧光性能进行表征。又以毕赤酵母作为指示生物,考察了延滞期的毕赤酵母分别与荧光碳点和量子点共培养后的生长曲线,并利用血球计数板法对培养至25h的毕赤酵母细胞进行计数。研究结果表明:荧光碳点对毕赤酵母的生长抑制作用不明显,即使在高浓度(14.4 mmol/L)下,也基本不影响毕赤酵母的生长;而CdTe量子点必须控制在很低的浓度(5.1μmol/L),才表现出低的细胞毒性。
Fluorescent carbon dots （CDs） were synthesized with glucose as the carbon source; CdTe quantum dots （QDs） were synthesized with mercapto succinic acid （MSA） as the stabilizing agent. Their fluorescent properties were characterized by UV absorption and fluorescence spectroscopy. The study result illustrated both of them had excellent optical performance. By using Pichia pastoris yeast as an indicator organism, the dependence of yeast＇ s growth with fluorescent CDs and fluorescent QDs at the lag period was also investigated, and Pichia pastoris yeast which cultured for 25 hours using blood corpuscle count plate method was counted. The study result illustrated fluorescent CDs had no influence on the growth of yeast, even the concentration of fluorescent CDs was as high as 14. 4 retool/L, while CdTe QDs showed low cytotoxicity only they were controlled at a very low concentration（5. 1 μmol/L）.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第11期1-4,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然基金资助项目(20875011)
辽宁省自然基金资助项目(20102221)
辽宁省教育厅资助项目(L2010542)
辽宁省教育厅杰出青年学者成长计划(LJQ2011109)
沈阳市科技局项目(F11-262-9-21)资助
关键词
荧光碳点
CDTE量子点
毕赤酵母
毒性
Fluorescent carbon dots
CdTe quantum dots
Pichia pastoris yeast
Cytotoxicity
