摘要
利用RACE技术从大豆品种‘阜豆11号’中克隆到1个ABC转运蛋白基因,该基因cDNA全长为4 693bp,其中开放读码框4 341bp,编码1 447个氨基酸,分子量162.5kD,具有高度保守的ATP结合位点,命名为GmABC。蛋白序列比对结果显示,该基因编码蛋白属于PDR(pleiotropic drug resistance)多向耐药性蛋白家族成员。系统进化树分析显示,GmABC与苜蓿PDR亲缘关系最近,相似性达84%。基因表达分析显示,GmABC受镉诱导表达,当Cd2+浓度达到100mg.L-1时,其表达量最高。研究表明,GmABC可能对阜豆镉胁迫抗性发挥重要作用。
In this study,a member of ABC transporter gene named GmABC,was isolated from Fuyang soybean（Fuyang Glycine max NO.11） using RACE（rapid amplification of cDNA ends） method.The full length of cDNA was 4 693 bp with a 4 341 bp open reading frame（ORF） which encoded a peptide of 1 447 amino acids and containing the highly conserved ATP binding site modules,and the predicted protein molecular mass was 162.5 kD.The protein sequence alignment showed that this gene encoded a protein belonging to the PDR（pleiotropic drug resistance） family of ABC proteins.The phylogenetic analysis showed that GmABC has the highest similarity with PDR from Medicago truncatula,which shared 84% homology with Medicago truncatula in the amino acid sequence.Expression analysis indicated that GmABC gene was induced by Cd2＋,expression level of GmABC gene was the highest at Cd2＋ concentration of 100 mg·L-1.The results suggested that GmABC may play an important role in Cd stress resistance of Fuyang soybean.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第9期1726-1730,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
安徽省教育厅自然科学基金项目(KJ2011B122)
关键词
阜豆
ABC转运蛋白
基因克隆
表达分析
Fuyang soybean ABC transporter gene isolation expression analysis
