摘要
采用PCR技术和气相色谱-质谱联用方法对西北农林科技大学葡萄酒学院保藏的50株酒酒球菌的arcABC基因簇进行检测,以确定其产生氨基甲酸乙酯的特性。结果显示:所有菌株均含有arcA、arcB和arcC基因,即arcABC基因簇;在添加精氨酸的模拟酒培养基中培养酒酒球菌30d后,通过气相色谱-质谱联用方法能够检测出微量的氨基甲酸乙酯;说明这50株酒酒球菌都具有产生氨基甲酸乙酯的能力。
PCR and GC-MS were used to detect arcABC gene cluster in fifty O. oeni strains conserved in the College of Enology of Northwest A＆F University to determine their abilities for ethyl carbamate production. All the O. oeni strains were found to possess the genes arcA, arcB and arcC, i.e. arcABC gene cluster. A trace amount of ethyl carbamate was found after 30 d of cultivation in a simulated wine culture medium fortified with arginine, indicating that all these strains have the potential to produce ethyl carbamate.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第19期227-230,共4页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD31B07)
中央高校基本科研业务费专项
国家现代农业产业技术体系建设专项(nycytx-30-ch-03)
