摘要
以2-甲酸甲酯苄磺酰胺和琥珀酸酐为原料合成半抗原(hapten),经薄层层析和1H-NMR鉴定后,采用混合酸酐法分别与牛血清蛋白(BSA)和卵清白蛋白(OVA)偶联获得免疫原(hapten-BSA)和包被原(hapten-OVA),紫外光谱分析法计算得其偶联比分别为8:1和13:1,初步说明具有较好免疫原性。通过动物免疫实验获得了效价达1:32000的阳性血清,采用间接酶联免疫吸附检测(ELISA)法检测抗体的IC50和IC10值为0.5032mg/L和0.0024mg/L,表明人工抗原合成成功。
In this study, a hapten was synthesized from methyl 2-[（sulfamoyl）methyl] benzoate and succinic anhydride. The synthetic hapten was identified by thin layer chromatography and 1H-NMR spectroscopy. An immune antigen （hapten-BSA） and a coating antigen （hapten-OVA） were prepared through separate conjugation of the hapten with BSA and OVA by mixed anhydride method. The conjugation ratios of the immune antigen and coating antigen were calculated to be 8：1 and 13：1 based on UV spectroscopic analysis, respectively, suggesting their good immunogenicity. Positive sera with a titer of 1：32000 were obtained by animal immunization trials. The IC50 and IC10 of antibody were determined by indirect ELISA assay to be 0.5032 mg/L and 0.0024 mg/L, respectively. Therefore, the synthesis of artificial antigen is successful.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第19期223-226,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2009BAD9B03-Z04)
