摘要
目的:优化超声波辅助提取双孢蘑菇多糖的工艺条件,评估双孢蘑菇多糖的抗氧化活性。方法:运用超声波辅助热水浸提、乙醇沉淀、Sevag法脱蛋白、乙醇再沉淀的方法提取双孢蘑菇多糖。在单因素(提取温度、提取时间、水料比及超声功率)实验基础上,运用4因素3水平的正交实验,对双孢蘑菇多糖的超声辅助提取参数进行优化。体外化学反应法测量双孢蘑菇多糖的抗氧化活性(还原潜力、清除超氧阴离子活性、清除过氧化氢活性)。结果:超声波辅助提取双孢蘑菇多糖的最优提取条件为提取温度65℃、提取时间40 min、液料比30 mL/g、超声功率170 W。此条件下,多糖的提取得率为5.6 014%。双孢蘑菇粗多糖产品中多糖的含量为75.48%。双孢蘑菇多糖体外具有一定的还原铁离子、清除超氧阴离子和清除过氧化氢能力,并且呈现出剂量-效应关系。结论:超声波辅助提取可以作为提取双孢蘑菇多糖的一种工艺方法;双孢蘑菇多糖具有一定的抗氧化活性,可作为一种天然的抗氧化剂被开发利用。
Objective： To optimize ultrasonic-assisted extraction parameters of polysaccharides from Agaricus bisporus and evaluate antioxidant activities of A.bisporus polysaccharides.Methods： Polysaccharides from A.bisporus was extracted by using methods of ultrasonic-assisted hot water lixiviation,ethanol precipitation,Sevag＇s deproteination and ethanol precipitation again.Extraction temperature,extraction time,ratio of water to raw material and ultrasonic power were selected in single-factor tests.Based on the single-factor tests,parameters combination for the ultrasonic-assisted extraction of A.bisporus polysaccharides was optimized by using four-factor-three-level orthogonal test.Antioxidant activities（reductive potential,superoxide anion scavenging activity and H2O2 scavenging activity） of A.bisporus polysaccharides were evaluated in vitro.Results： Optimum conditions for the extracting of A.bisporus polysaccharides were extracting temperature 65 ℃,extracting time 40 min,ratio of water to raw material 30 mL/g and ultrasonic power 170 w.Practicing this optimal condition,extraction yield of polysaccharides from A.bisporus was 5.6 014%.In crude polysaccharides of A.bisporus,carbohydrates content,determined by applying the phenol-sulfuric acid method,was 75.48%.Polysaccharides of A.bisporus could reduce ferric ion,scavenge superoxide anion and hydrogen peroxide in a dose-dependent manner.Conclusion： Utrasonic-assisted extraction could be used in the extracting of A.bisporus polysaccharides.Polysaccharides of A.bisporus,had direct and potent antioxidant activities,might be developed and utilized as natural antioxidant.
出处
《中药材》
CAS
CSCD
北大核心
2012年第8期1340-1347,共8页
Journal of Chinese Medicinal Materials
基金
安徽省教育厅自然科学研究项目(KJ2010B256)
关键词
双孢蘑菇
多糖
超声波
提取
抗氧化
Agaricus bisporus
Polysaccharides
Ultrasonic wave
Extraction
Antioxidation
