摘要
目的:建立同时测定生地黄中梓醇和毛蕊花糖苷含量的方法;采用星点设计-效应面法优化生地黄中梓醇和毛蕊花糖苷的回流提取工艺。方法:使用波长切换的方法检测梓醇和毛蕊花糖苷的含量,并考察乙醇浓度及液料比对梓醇和毛蕊花糖苷提取效率的影响。通过对自变量的总评归一值进行多元线性回归,采用效应面优化最佳的工艺条件,并进行预测分析。结果:梓醇进样量在0.047～3.00μg、毛蕊花糖苷进样量在0.016～1.00μg范围内其单位浓度与峰面积积分值呈良好线性关系;确定回流提取工艺条件为乙醇浓度(A):38.0%;液料比(B):19.3mL/g。工艺验证的实测值与总评归一预测值基本吻合,二项式拟合的相关系数R2=0.7498(P<0.05)。结论:所建生地黄中梓醇和毛蕊花糖苷含量测定的方法准确度、精密度和重现性均良好;采用星点设计-效应面法优选方法优化的地黄提取工艺,方法简便、且有较优的预测性。
Objective：To establish a method for simultaneously quantification of the Catalpol and Acteoside from Rehmanniae Radix and optimize the extraction process from Rehmanniae Radix by response surface methodology（RSM） using central composite design（CCD）.Methods： The effect of ethanol concentration and the solvent-material ratio of the extraction were investigated on the overall extraction efficiency.Design Expert was used for regression analysis and determination coefficient of the regression equation.Results： A good linear relationship was obtained from 0.047～3.00 μg and 0.016～1.00 μg for Catalpol and Acteoside,respectively.The optimal condition of extraction process was as follows： raw material was extracted with 19.3 mL/g solvent-material ratio of 38.0% ethanol.The R-Squared of binomial fitting of the complex model was 0.7498（P0.05）.The experimental values were accordance with values obtained from model prediction.Conclusion： The accuracy and precision of the developed HPLC protocol is adequate for simultaneous quantification of Catalpol and Acteoside in Rehmanniae Radix.The optimized extraction condition could be obtained by RSM using CCD.
出处
《中药材》
CAS
CSCD
北大核心
2012年第8期1318-1322,共5页
Journal of Chinese Medicinal Materials
基金
澳门科学技术发展基金(FDCT044/2011/A2)
关键词
地黄
梓醇
毛蕊花糖苷
提取工艺
星点设计
波长切换
Rehmanniae Radix
Catalpol
Acteoside
Extraction process
Central composite design
Switching wavelengths
