摘要
目的:建立夏枯草药材的指纹图谱,为夏枯草药材的质量控制提供依据。方法:采用HPLC法,AgilentEclipse XDB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-1.0%醋酸水为流动相梯度洗脱,流速为1.0 mL/min,检测波长290 nm,柱温30℃,进样10μL。结果:建立了19批夏枯草药材的指纹图谱,有14个共有峰,多数峰可以达到较好分离,具有较高的相似度。结论:建立的HPLC指纹图谱有较好的精密度、重复性和稳定性,可以作为夏枯草质量评价主要依据之一。
Objective：To establish HPLC fingerprint of Prunella vulgaris and provide evidence for quality control and identification of the Chinese crude drug.Methods： The HPLC method was used,chromatography conditions were Agilent Eclipse XDB-C18（4.6 mm×250 mm,5 μm）column with gradient mobile phase of acetonitrile-0.1% acetic acid,UV detection wavelength was 290 nm and the column temperature was 30 ℃ with the flow rate of 1.0 mL/min,the sample injection was 10 μL.Results：HPLC fingerprints of 19 samples of Prunella vulgaris were established.14 common peaks were selected as the fingerprint peaks in all samples.Among the obtained fingerprints,most of the detected peaks were separated effectively.19 samples had high similarities.Conclusion：The established HPLC fingerprint has desirable accuracy,repeatability and stability,which can be used for one of the quality control methods of Prunella vulgaris.
出处
《中药材》
CAS
CSCD
北大核心
2012年第8期1234-1237,共4页
Journal of Chinese Medicinal Materials
关键词
夏枯草
HPLC
指纹图谱
质量控制
Prunella vulgaris L.
Fingerprints
HPLC
Quality control
