摘要
目的:从不同发育阶段的霍山石斛组织培养物中提取分离多糖,比较多糖的理化性质和免疫活性。方法:采用水提醇沉法提取多糖,阴离子交换层析法分离多糖,分光光度法、GC-MS、IR、荧光电泳分析多糖的理化性质,ELISA法测定多糖的免疫活性。结果:霍山石斛不同发育阶段培养物中多糖含量无显著差异,经阴离子交换层析均得到5种多糖组分,且都是由葡萄糖、半乳糖和甘露糖组成,IR和荧光电泳分析显示它们的化学结构特性无显著变化;体外实验表明,霍山石斛不同发育阶段培养物多糖具有相似的促进小鼠脾细胞产生IFN-γ和TNF-α的作用。结论:组织快繁中霍山石斛不同发育阶段培养物中多糖的理化性质和免疫活性无显著差异,表现出一定的稳定性。
Objective： To compare the physicochemical properties and immun activities of polysaccharides extracted from cultures at different development stages of Dendrobium huoshanense.Methods： Polysaccharides were extracted by hot water and alcohol precipitation,and isolated by anion exchange chromatography.The physicochemical properties of polysaccharides were analyzed by spectrophotometry,GC-MS,IR and fluorophore-assisted carbohydrate electrophoresis（FACE）.ELISA was used to determine the immun activities of polysaccharides.Results： The polysaccharides from cultures at different development stages of D.huoshanense had no obvious discrepancy in contents.5 polysaccharides were isolated by anion exchange chromatography with the same elution conditions.GC-MS analysis showed that the polysaccharides from cultures at different development stages of D.huoshanense consisted of glucose,mannose and galactose.IR and FACE displayed that different polysaccharides had no significant change in chemical properties.In vitro experiments with spleen cells proved that the polysaccharides from cultures at different development stages had similar effects on stimulating IFN-γ and TNF-α releases.Conclusion： No significant differences are found in the physico-chemical properties and immun activities of polysaccharides extracted from cultures at different development stages of D.huoshanense,suggesting that the polysaccharides of D.huoshanense have stability in quality to some extent during tissue culture.
出处
《中药材》
CAS
CSCD
北大核心
2012年第8期1195-1199,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(20872024)
关键词
霍山石斛
组织快繁
多糖
理化性质
免疫活性
Dendrobium huoshanense Tang at Cheng
Tissue culture
Polysaccharides
Physicochemical property
Immun activity
