摘要
目的建立人尿液中4种多环芳烃(polycyclic aromatic hydrocarbons,PAHs)代谢产物的液相色谱-质谱(LC-MS-MS)同时测定法。方法尿液经过酶解,固相萃取净化富集,旋转蒸发浓缩,采用LC-MS-MS法检测尿中1-羟基芘、2-羟基芴、6-羟基屈、3-羟基苯并芘的浓度。结果在0.1～10μg/L的线性范围内,1-羟基芘、2-羟基芴、3-羟基苯并芘、6-羟基屈所得的回归方程均呈较好的线性关系(r≥0.999);该方法的检出限分别为0.2、0.15、0.2、0.2μg/L,该方法的平均回收率为61.3%～100.7%,RSD为1.5%～9.7%。结论该方法灵敏度高,重现性和回收率好,特异性高,适用于人尿样中PAHs单羟基代谢物的快速、大批量检测。
Objective To develop a method for simultaneous determination of four metabolites of polycyclic aromatic hydrocarbons （OH-PAHs） in human urine （1-hydroxypyrene, 2-hydoxyfluorene, 6-hydroxychrysene, and 3-hydoxybenz[a] pyrene） by liquid chromatography-tandem mass spectrometry （LC-MS-MS） method. Methods The samples were hydrolyzed by enzyme first, then were purified and enriched by solid phase extraction, concentrated and determined with LC-MS-MS. Results The regression equations showed a good linearity in the range of 0.1-100 ng/ml and the correlation coefficients were not less than 0.99. The limits of detection of the method were 0.18-0.3 ng/ml. The rates of recovery were 61.3%-100.7% and the relative standard deviations were 1.5%-9.7%. Conclusion The established method is sensitive, fast and reproducible with higher precision and better recoveries, it is applicable to simultaneous determination of polycyclic aromatic hydrocarbons in human urine.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2012年第10期933-936,共4页
Journal of Environment and Health
基金
国家自然科学基金(81160337)
新疆教育厅青年教师科研培育基金(XJEDU2011S23)
关键词
色谱法
液相
串联质谱
多环芳烃代谢物
尿液
Chromatography, liquid
Tandem mass spectrometry
Metabolites of polycyclic aromatic hydrocarbon
Urine
