摘要
目的探讨消栓颗粒对缺氧缺血性脑损伤(HIBD)的治疗作用及其机制。方法 7日龄SD新生大鼠36只,随机分为假手术组、HIBD模型组和消栓颗粒治疗组,每组12只。HIBD模型组及消栓颗粒治疗组大鼠建立HIBD模型,消栓颗粒治疗组灌服消栓颗粒(8 g·kg-1,每日1次),假手术组及HIBD模型组灌服等量9 g·L-1盐水。于造模第14天取其脑组织制作石蜡切片,HE染色光镜下观察3组大鼠左侧大脑半球病理形态学的变化,用免疫组织化学法观察Bcl-2、Bax蛋白在各组大鼠海马及皮质区的表达。结果 1.病理形态学观察:假手术组大鼠左侧脑组织未见明显病变,HIBD模型组大鼠可见弥散性变性、坏死的神经细胞,消栓颗粒治疗组偶见坏死的神经细胞。病理学评分:假手术组、HIBD模型组及消栓颗粒治疗组分别为22.58±2.61、50.67±2.27、32.17±1.40,3组间两两比较差异均有统计学意义(Pa<0.01)。2.免疫组织化学染色:假手术组、HIBD模型组及消栓颗粒治疗组大鼠左侧脑组织Bcl-2阳性细胞数分别为10.52±2.70、22.63±3.17、41.38±2.09,3组间两两比较差异均有统计学意义(Pa<0.01);假手术组、HIBD模型组及消栓颗粒治疗组大鼠左侧脑组织Bax阳性细胞数分别为22.15±9.73、56.71±28.04、49.63±18.52,3组间两两比较差异均有统计学意义(Pa<0.01)。结论消栓颗粒可减轻HIBD新生大鼠的脑损伤,其机制可能与其上调Bcl-2蛋白、下调Bax蛋白的表达有关。
Objective To explore the effect of Xiao Shuan Ke Li （XSKL） on neonatal rats with hypoxic - ischemic brain damage （HIBD） and its mechanism. Methods Thirty - six seven - day - old Sprague Dawley （SD） rats were randomly divided into 3 groups ： sham operation group（ n = 12 ） , HIBD model group （n = 12） and XSKL treatment group（ n = 12）. Observation group and model group were induced to establish animal models with HIBD. By the microscopic magnification, the left common carotid artery was doubly ligated with 5 - 0 silk, and then sheared, after that all rats were laid back to their mothers for 2 h recovery. Then the rats were placed into hypoxia chamber in the environ-ment of 37 ℃ for 2.5 h,with a continuous flow of 80 mL · L^-1 02 ＋ 920 mL · L^-1 N2 delivered at 2.5 mL · L^-1. While in the sham opera-tion group were only the left common carotid was dissociated without ligation or exposed to hypoxia. The rats of XSKL treatment group were given oral XSKL（8 g · kg^-1 , qd） for 14 d, HIBD model group and sham operation group were given the same amounts of 9 g · L^-1 saline for 14 d. The pathomorphology changes in the left brain were observed under the light microscope. The expressions of Bcl - 2 and Bax protein in left brain were measured by immunohistoehemieal staining. Results 1. Degeneration and necrosis were found obviously in the left brain in the HIBD model group ,while degeneration and necrosis were found less in XSKL treatment group and rarely in sham operation group. The patho-logical scores ： sham operation group, HIBD model group and the XSKL treatment group were 22.58 ± 2.61,50.67 ± 2.27,32.17 ± 1.40,re- spectively ,and each other showed significant differences （Pa 〈 0.01 ）. 2. Immunohistochemical staining results showed that the amounts of masculine cells of Bcl - 2 in the left brain was few in the sham operation group （ 10.52 ± 2.70 ）, the masculine cells of Bcl - 2 in XSKL treat- ment group （41.38 ± 2.09） were much more than that in HIBD model group （22.63 ± 3.17 ）, and each other showed significant differences （Pa 〈 0.01 ）. The masculine cells of Bax in the left brain were few in the sham operation group（ 22.15 ± 9.73 ） ,the masculine cells of Bax in XSKL treatment group（49.63 ± 18.52） were much fewer than that in HIBD model group（56.71 ± 28.04）（ P 〈 0.01 ）, and each other showed significant differences （Pa 〈 0.01 ）. Conclusions XSKL can reduce the brain damage in neonatal rats with HIBD, the mechanism might be that XSKL can up - regulate Bcl - 2 protein and down - regulate of Bax protein.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2012年第20期1603-1606,共4页
Journal of Applied Clinical Pediatrics
