摘要
目的:观察复方芪珠方的不同提取物对大鼠肝脏肿瘤坏死因子(TNF-α)、白介素-4(IL-4)、白介素-8(IL-8)、白介素-10(IL-10)表达的影响,探讨复方芪珠方抗肝纤维化的作用机制。方法:70只大鼠随机分为空白、模型组、复方芪珠方水提高、低剂量组、复方芪珠方醇提高、低剂量组,秋水仙碱组。除正常组外,其余各组大鼠皮下注射40%四氯化碳(CCl4)橄榄油造模,首次5 mL.kg-1,以后每次3 mL.kg-1,每周2次,共8周。于造模开始次日给药,复方芪珠方高、低剂量组16.2,9.72 g.kg-1ig,秋水仙碱组0.5 mg.kg-1ig,每日1次,连续8周。造模结束后所有大鼠处死,肝脏组织学观察,测定肝脾指数,酶联免疫吸附试验(ELISA)技术检测TNF-α,IL-4,IL-8,IL-10在肝内的表达。结果:模型组大鼠肝组织炎症程度及纤维化程度均高于正常组,且肝组织中TNF-α,IL-4,IL-8的表达也明显增加,IL-10的表达则显著减少;应用复方芪珠方后,肝组织炎症和纤维化程度降低,同时相对模型组,肝组织中TNF-α,IL-4,IL-8的表达减少,IL-10的表达增加。结论:复方芪珠方用于大鼠肝纤维化有显著效果,其机制可能与其能影响TNF-α,IL-4,IL-8,IL-10等细胞因子在肝组织的表达有关。
Objective： To investigate the effect of Compound Qizhu tablet on the expression of tumor necrosis factor -a （TNF-a） , interleukin-4 （IL-4） , interleukin-8 （IL-8） and Interleukin-10 （IL-10） in rats with liver fibrosis. Method： Seventy SD rats were randomly divided into normal group, model group, Qizhu high-dose group （water preparation）, Qizhu low dose group （water preparation）, Qizhu high-dose group （ethanol preparation） , Qizhu low dose group （ethanol preparation） , and colchicine group. All the rats except normal group received 40% CC14 subcutaneous injection twice a week for 8 weeks, rats was firstly given 40% CC14 （5 mL· kg^（-1）） and 3 mL .kg-lsecondly. Rats in both Qizhu high-dose group and small dose group were ig given 16.2, 9.72 g ·kg^（-1） Qizhu decoction respectively, while rats in colchieine group were fed with 0.5 mg·kg^（-1） colchicine. Liver histology and liver and spleen index were observed after the modelling. The expression of TNF-a, IL-4, IL- 8 and IL-10 was detected by enzyme linked immune sorbent assay （ELISA）. Result： The degree of hepatic fibrosis in all treatment groups was obviously reduced compared with model group. Compared with normal group, the expression of TNF-R, IL-4 and IL-8 in model group was elevated significantly, whereas the expression of IL-10 / was decreased （P 〈0.01 ）. Compared with modelfgroup, the expression of TNF-a, IL-4 and IL-8 in treatment groups were decreased significantly, whereas the expression of IL-10 was increased （P 〈 0.01 ）. Conclusion： Compound Qizhu tablet has obvious therapeutic effect on rats with liver fibrosis. The mechanism may be correlated to interfering the disturbance of some cytokines such as TNF-R, IL-4, IL-8 and IL-10.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第21期218-221,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2008ZX10005-009)
江苏省研究生培养创新工程项目
