摘要
目的研究重组结核分枝杆菌ESAT6-CFP10蛋白对临床常见致病分枝杆菌及常见环境分枝杆菌致敏豚鼠的皮试反应。方法以鸟分枝杆菌、胞内分枝杆菌、蟾蜍分枝杆菌、堪萨斯分枝杆菌、海分枝杆菌、瘰疠分枝杆菌、戈登分枝杆菌、苏加分枝杆菌、龟分枝杆菌脓肿亚种、偶发分枝杆菌、草分枝杆菌、微黄分枝杆菌、非洲分枝杆菌、牛分枝杆菌、结核分枝杆菌与卡介菌等16株菌株的活菌菌液分别经腹股沟皮下攻击豚鼠,每组5只,3～4周后,分别皮内注射TB-PPD、PPD-B和重组结核分枝杆菌ESAT6-CFP10蛋白各0.1ml,于注射后24、48h观察局部硬结的纵径与横径,根据48h的反应结果进行判定。结果PPD-B对16种分枝杆菌活菌致敏豚鼠的皮试硬结直径均≥5mm,呈阳性,阳性比例均为5/5;TB-PPD对结核、牛、非洲分枝杆菌和卡介菌等活菌致敏豚鼠的皮试硬结直径均≥5mm,呈阳性,阳性比例均为5/5;重组结核分枝杆菌ESAT6-CFP10蛋白对结核、牛和非洲分枝杆菌活菌致敏豚鼠的皮试硬结直径均≥5mm,呈阳性,阳性比例均为5/5;而对鸟、胞内、蟾蜍、堪萨斯、海、瘰疠、戈登、苏加、龟脓、偶发、草、微黄和卡介菌等分枝杆菌活菌、结核分枝杆菌死菌致敏豚鼠的皮试反应均呈阴性,阳性比例均为0/5。结论重组结核分枝杆菌ESAT6-CFP10蛋白能提高结核分枝杆菌感染诊断的特异度并能区别结核分枝杆菌是否存活、卡介菌致敏导致的皮试超敏反应。
Objective To study skin test with Mycobacterium tuberculosis recombinant ESAT6-CFP10 protein in Guinea pigs sensitized with several clinical common pathogenic mycobacteria and environmental mycobacteria.Methods Guinea pigs were infected by subcutaneous inguinal injection with 16 kinds of live mycobacterial liquids,respectively.There were 5 Guinea pigs each group.After 3 to 4 weeks,the Guinea pigs were injected intradermally with 0.1 ml of TB-PPD,PPD-B and the recombinant protein ESAT6-CFP10,respectively.The longitudinal and transverse diameters of local indurations were observed at 24 h and 48 h after the injection.The skin test were determined as positive result when average diameter was ≥5 mm at 48 h after the injection.Results In PPD-B group,the skin tests of Guinea pigs sensitized with 16 kinds of mycobacteria were all positive.In TB-PPD group,the skin tests of Guinea pigs sensitized with M.tuberculosis,M.bovis,M.africanum,M.bovis BCG were also positive.In ESAT6-CFP10 group,the skin tests of Guinea pigs sensitized with M.tuberculosis,M.bovis and M.africanum were positive,other Guinea pigs sensitized were negative.Conclusion Mycobacterium tuberculosis recombinant ESAT6-CFP10 protein can improve the specificity of Mycobacterium tuberculosis infection diagnosis.
出处
《中国防痨杂志》
CAS
2012年第10期676-680,共5页
Chinese Journal of Antituberculosis
