摘要
以1/2Hoagland溶液培养的阜豆幼苗根系为对照群体,含Cd2+浓度为100μM营养液处理的为目标群体,进行抑制差减杂交。用经过对照组cDNA差减目标组cDNA构建了一个含有大约600个独立克隆的差减文库。随机挑取部分克隆进行菌落PCR鉴定,表明插入的片段大小均在250～800 bp。对已确认的6个阳性克隆测序,序列分析和同源性比较,表明它们与镉胁迫有关。
A subtracted cDNA library of Fuyang soybean seedlings roots specific to Cd^2＋ stress was constructed by suppression subtractive hybridization （SSH）. It was composed of approximately 600 individual recombinant clones. SSH was performed between two groups of Fuyang soybean seedlings, the group of Fuyang soybean seedlings cultivated in 1/2 Hoagland＇Ls （H） solution as driver and the other treated by Cd^2＋ stress of 1/2 Hoagland＇Ls solution as tester. The recombinant clones from the subtracted cDNA library were chosen randomly and PCR identification of colonies was performed. Result showed that insertions were ranged from 250 to 800bp. Six confirmed positive clones were sequenced. It indicated that they were closely related to cadmium stress.
出处
《生物学杂志》
CAS
CSCD
2012年第5期15-18,共4页
Journal of Biology
基金
安徽省教育厅自然科学基金项目(KJ2011B122)
关键词
差减杂交
阜豆
镉
诱导表达
subtractive hybridization
Fuyang soybean
Cd^2 ＋
induction expression
