期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Bdf1p转录因子BD2和ET结构域双缺失对盐胁迫诱导酵母细胞凋亡的影响 被引量：1

Effect of a double deletion of BD2 and ET domains of Bdf1p transcription factor 1 on the salt-induced yeast apoptosis
在线阅读 下载PDF
导出
摘要 以酿酒酵母为研究材料,分析Bdf1p转录因子BD2和ET结构域双缺失对盐胁迫诱导细胞凋亡的影响。研究结果表明,盐胁迫条件下,与野生型酵母菌株相比,Bdf1p转录因子BD2和ET结构域双缺失体BBDΔ,表现为细胞存活率下降、活性氧升高、细胞核裂解、磷脂酰丝氨酸外翻、细胞凋亡程度加剧,表明Bdf1p转录因子BD2和ET结构域在盐胁迫诱导的酵母细胞凋亡中发挥重要作用。 The paper investigated the effect of a of BD2 and ET domains of Bdflp transcription factor 1 on the salt-induced apoptosis in Saccharomyces cerevisiae. The results showed that the Bdflp BD2 and ET domains double deletion mutant BBDA displayed more serious salt-induced apoptosis compared with wild type strain under salt stress conditions, including more accumulation of reactive oxygen species, chromatin fragmentation and losing of plasma membrane asymmetry. The data indicated that the BD2 and ET domains of Bdflp was important for salt-induced yeast apoptosis.
作者 刘向勇 张小华 刘乃国 武玉永
机构地区 滨州医学院药学院
出处 《广东农业科学》 CAS CSCD 北大核心 2012年第18期167-169,共3页 Guangdong Agricultural Sciences
基金 国家自然科学基金(31000039) 山东省高校科技计划项目(J09LC08)
关键词 酿酒酵母 Bdf1p 盐胁迫 细胞凋亡 Saccharomyces cerevisiae Bdflp salt stress apoptosis
分类号 TS261.11 [轻工技术与工程—发酵工程]
  • 相关文献

参考文献9

  • 1付畅,杨传平,刘桂丰,姜静.酵母耐盐机制的研究进展[J].遗传,2003,25(6):757-761. 被引量：18
  • 2Garcidaeblas B, Rubio F, Quintero F J,et al. A Differentialexpression of two genes encoding isoforms of the ATPaseinvolved in sodium efflux in Saccharomyces cerevisiae [J].MolGen Genet, 1993, 236:363-368.
  • 3Martinez P, Perssion B L. Identification, cloning andcharacterization of a derepressible Na .-coupled phosphatetransporter in Saccharomyces cerevisiae[J]. Mol Gen Genet,1998,258(6):628-638.
  • 4Chua P, Roeder G S. Bdfl, a yeast chromosomal proteinrequired for sporulation[J].Mol Cell Biol, 1995,15(7):3685-3696.
  • 5Sawa C, Nedea E,Krogan N, et al. Bromodomain factorl(Bdfl)is phosphorylated by protein kinase CK2[J]. Mol Cell Biol,2004,24(11): 4734-4742.
  • 6Xingyong L, Hui Y, Xiaohua Z, et al. Bdflp deletion affectsmitochondrial function and causes apoptotic cell death undersalt stress[J]. FEMS Yeast Res,2009, 9(2):240-246.
  • 7Honmann S, Willem H M. Yeast Stress Responses [M].Berlin:Springer,2003:121 -229.
  • 8Wadskogi, Maldener C,Proksch A, et al.Yeast lacking the SR07/S0P1 -encoded tumor suppressor homologue show increasedsusceptibility to apoptosis -like cell death on exposure to NaClstress[J]. Mol Biol Cell, 2004,15(3):1436-1444.
  • 9Hug G H, Damsz B, Matsumoto T K,et al. Salt causes iondisequilibrium-induced programmed cell death in yeast and plants[J].Plant J, 2002,29(5):649-659.

二级参考文献1

共引文献17

同被引文献18

  • 1Lesage G, Bussey H. Cell wall assembly in Saccharomyces cerevisiae[ J ]. Microbiol Mol Biol Rev, 2006, 70 ( 2 ) : 317-343.
  • 2Levin D E. Regulation of cell wall biogenesis in Saccharo- myces cerevisiae:The cell wall integrity signaling pathway [J]. Genetics,2011,189(4) :1145-1175.
  • 3Ram A F, Klis F M. Identification of fungal cell wall mu- tants using susceptibility assays based on Calcofluor white and Congo red[ J]. Nat Protoc ,2006,1:2253-2256.
  • 4Wach A. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruption in S. cere- visiae[J]. Yeast, 1996,12(3 ) :259-265.
  • 5Bonawitz N D, Rodeheffer M S, Shadel G S. Defective mitnchondrial gene expression results in reactive oxygen species-mediated inhibition of respiration and reduction of yeast life span [J]. Mol Cell Biol,2006,26(13): 4818-4829.
  • 6Madco F, Herker E, Maldener C, et al. A caspase-related protease regulates apoptosis in yeast [ J ]. Mol Cell, 2002,9(4) :911-917.
  • 7Levin D E. Cell wall integrity signaling in Saccharomyces cerevisiae [ J 1. Microbiol Mol Biol Rev, 2005,69 ( 2 ) : 262-291.
  • 8Madeo F, Fr6hlich E, Fr6hlich K U. A yeast mutant showing diagnostic markers of early and late apoptosis [Jl. J Cell Bio1,1997,139(3) :729-734.
  • 9Sharon A, Finkelstein A, Shlezinger N, et al. Fungal apo- ptosis : Function, genes and gene function [ J ]. FEMS Yli- crobiol Rev ,2009,33 (5) :833-854.
  • 10Barhoom S, Sharon A. Bcl-2 proteins link programmed cell death with growth and morphogenetic adaptations in the fungal plant pathogen Colletotrichum gloeosporioides [ J ]. Fungal Genet Biol, 2007,44 ( 1 ) : 32-43.

引证文献1

二级引证文献8

广东农业科学
2012年 第18期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部