摘要
目的:观察、比较巴戟天、鹿角胶、淫羊藿、骨碎补对体外培养SD大鼠骨髓间充质干细胞(BMSCs)促增殖的影响,探讨其作用机理。方法:建立SD大鼠BMSCs体外培养体系,倒置显微镜、苏木精-伊红染色进行形态学观察;流式细胞仪(FCM)进行BMSCs细胞周期分析;MTT法检测BMSCs生长曲线;4味温阳补肾药含药血清配制,确定其最佳药物浓度和最佳血清浓度对BMSCs促增殖的影响。结果:体外培养大鼠BMSCs经倒置显微镜、苏木精-伊红染色的形态学观察均符合BMSCs的形态及生长特点。高、中、低剂量的四味中药含药血清对F3BMSCs 24、48、72h的增殖效应显示,以中剂量组20%含药血清对F3BMSCs 48h后促增殖最明显,其组间比较结果为淫羊藿>骨碎补>鹿角胶>巴戟天。结论:巴戟天、鹿角胶、淫羊藿、骨碎补四味温阳补肾药均有促BMSCs增殖作用。淫羊藿促BMSCs增殖效果最佳,其组间比较结果为淫羊藿>骨碎补>鹿角胶>巴戟天。
Objective：To observe and compare the proliferation-promoting effects of Morinda Officinalis How,Colla Cornus Cervi,Rhizoma Drynariae and Herba Epimedii on bone marrow mesenchymal stem cells（BMSCs） in vitro and to investigate the possible mechanism.Methods：The in vitro culture system of SD rat BMSCs was established.The morphology was observed by inverted microscope and HE staining.MTT assay was used for grow curve.Rat serum containing Chinese medicine of warming kidney yang was prepared.Results：The inverted microscope and HE staining showed that the rat BMSCs culture in vitro were in accordance with BMSCs morphology and growth characteristics.The high,medium and low doses of those four Chinese medicine rat serum promoted proliferation at 24h,48h,and 72h,with 20% medium dosage serum group at 48h showing the best promotion effect.The promoting effects from the best to the less effective were Rhizoma Drynariae,Herba Epimedii,Colla Cornus Cervi,and Morinda Officinalis How,respectively.Conclusion：Morinda Officinalis How,Colla Cornus Cervi,Rhizoma Drynariae and Herba Epimedii were effective in promoting proliferation of BMSCs,among which has the best proliferation-promoting effect.
出处
《中国中医骨伤科杂志》
CAS
2012年第10期1-4,8,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金(项目编号:30973763
81173283)
教育部博士点基金(项目编号:20093519110001)
关键词
巴戟天
鹿角胶
淫羊藿
骨碎补
骨髓间充质干细胞
增殖
Morinda Officinalis How
Colla Cornus Cervi
Herba Epimedii
Rhizoma Drynariae
Bone marrow mesenchymal stem cells
Proliferation
