HBV-ACLF患者外周血和肝脏NK细胞的表达频率及功能与肝损伤的相关性被引量：2

Association of the frequency and function of peripheral and liver natural killer cells with liver injury in HBV-ACLF patients

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摘要目的探讨HBV相关慢加急性肝衰竭（HBV-ACLV）患者外周血和肝组织自然杀伤（NK）细胞的表达频率及功能与肝损伤的相关陛。方法利用多色流式技术检测14例HBV-ACLF患者和15例HBV-CHB（慢性乙型肝炎）患者外周血NK细胞（CD3-CD56＋）的表达频率和NK细胞表面CD10Ta的表达，胞内细胞因子染色技术检测NK细胞分泌IFN-γ的水平。收集20例HBV-ACLF患者、10例轻度CHB患者肝组织穿刺标本，利用免疫组织化学双染技术检测肝组织中CD3和CD57抗原的表达。分析轻度CHB和HBV-ACLF患者肝脏NK细胞的表达频率及与肝损伤程度（TBIL水平）的相关性。结果HBV-ACLF患者与轻度CHB患者外周血NK细胞的表达频率和IFN-γ的表达差异均无统计学意义（12．7％±5．3％vs9．6％±3．3％和4．52％±2．52％vs9．06％±7．6％，P均〉0．05），HBV-ACLF患者外周血CD107a表达（NK细胞的杀伤活性）要显著强于轻度CHB患者（52．1％±18．9％vs30．9％±12．4％，P〈0．05）。HBV-ACLF患者肝组织中CD57＋NK细胞的表达频率显著高于轻度CHB患者（95．1±21．3／低倍镜视野V89．5±10．6／低倍镜视野，P〈0．01）。肝脏CD57＋NK细胞的表达频率与TBIL水平显著正相关，相关系数为0．936。结论外周血NK细胞杀伤活性显著增强和肝脏中CD57＋NK细胞大量募集可能是导致HBV-ACLF免疫性肝损伤加剧，肝细胞大量凋亡和坏死的重要因素。 Objective To investigate whether the expression frequency and function of peripheral and liver NK cells was correlated with the liver injury in patients with hepatitis B virus related acute-on-chro- nic liver failure（HBV-ACLF）. Methods Peripheral blood samples were obtained from fifteen HBV-ACLF patients and fifteen chronic hepatitis B patients. The frequency of peripheral NK cells （ CD3- CD56＋） and CD107a expression on surface of peripheral NK cells were detected by multicolor flow cytometry. Expression of IFN-γby peripheral NK ceils was detected by intracellular cytokine staining. Needle biopsy liver tissues were obtained from twenty patients with HBV-ACLF, ten patients with mild CHB, and expression of live NK ceils （ CD3-CD57＋） was analyzed by dual immmunohistochemical staining of CD 3 and CD57 . Results There was no significant difference in the frequency of peripheral NK cell and IFN-γexpression by peripheral NK cells between HBV-ACLF and mild CHB patients. However, CD107a expression on surface of peripheral NK calls of HBV-ACLF patients was remarkably higher than that of CHB patients. The frequency of hver CD57＋NK cell of HBV-ACLF patients was remarkably higher than that of CHB patients （95.1±21.3/low power field vs 9.5±10.6/low power field, P〈0. 01 ）. Further analysis revealed that the frequency of liver CD57＋ NK cells in HBV-ACLF patients was positively correlated with the TBIL level. Conclusion The en- hanced cytotoxic activity of peripheral NK cells and the recruitment of liver CD57＋ NK cells may aggravate immune-mediated liver injury and promote the apoptosis and necrosis of hepatocytes.

作者邹勇周耀勇宋戈

机构地区中山大学附属第三医院输血科中山大学附属第六医院感染科华中科技大学同济医学院附属同济医院感染免疫研究室

出处《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2012年第7期636-641,共6页 Chinese Journal of Microbiology and Immunology

基金国家自然科学青年基金资助项目（81000180）

关键词乙型肝炎病毒肝衰竭自然杀伤细胞 Hepatitis B virus Liver failure Natural killer cell

分类号 R575.3 [医药卫生—消化系统]

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