摘要
目的连续1年动态观察低浓度苯作业工人外周血T细胞亚群及外周血细胞水平，探索苯暴露后T细胞功能受损与苯致造血功能损伤的关系。方法选取广州某涂料厂新人苯作业车间工人88人（男58人，女30人，年龄18—22岁）为观察组，每4个月检测1次外周血T淋巴细胞亚群相对百分比及外周血细胞计数，共检测4次，同时检测车间工人作业点苯浓度。非苯作业车间工人88人作为对照组，男58人，女30人，年龄18～25岁。结果第1次和第2次检测苯接触工人外周血细胞计数无明显改变，第3、4次检测WBC[（6．4±3．0）×109／L、（6．3±2．7）×109／L]下降，第4次检测PLT[（179±74）×109/L]下降，与对照组[WBC：（7．3±3．0）×109/L，PLT：（189±70）×109／L]比较，差异均有统计学意义（P〈0．05）。第3次检测观察组外周血CD3＋T细胞百分比上升，第2、3、4次检测观察组外周血T细胞百分比[（50．77％±11．05％）、（45．40％±9.41％）、（41．27％±10．62％）]逐渐下降，CD8＋T细胞百分比[（46．07％±10．18％）、（50．36％±10．62％）、（56．40％±9．41％）]逐渐上升，与对照组[CD4＋：（54．29％±12．78％），CD8＋：（37．25％±12．30％）]比较，差异均有统计学意义（P〈0．05）。结论低浓度苯作业工人T淋巴细胞亚群的改变早于血液系统的改变。
Objective To observe the T cell subsets and blood cells in the peripheral blood of workers exposed to low levels of benzene for one year, and to investigate the relationship between T cell function impairment and benzene-induced hematopoietic injury after benzene exposure. Methods Eighty-eight workers （58 males and 30 females, aged 18-22 years） who just began to work in the workshop of a paint factory with exposure to benzene in Guangzbeu, China were assigned to experimental group, and 88 workers （58 males and 30 females, aged 18-25 years） who worked in the workshop without exposure to benzene were selected as controls. The blood samples of the workers were examined once every 4 months to measure the percentages of peripheral T cell subsets and peripheral blood cell counts in the one-year study. The benzene concentrations at operation points were also measured. Results The peripheral blood cell counts in the benzene-exposed workers had no significant changes in the first and second examinations; the white blood cell （WBC） counts in the experimental group in the third and fourth examinations were significantly lower than that in the control group [（6.4±3.0） ×109/L and （6.3±2.7）×109/L vs （7.3±3.0）×109/L, P〈0.05], and the platelet （PLT） count in the experimental group in the fourth examination was also significantly lower than that in the control group [（179±74） ×109/L vs （189±70）×109/L, P〈0.05]. Compared with those in the control group （CD4＋： 54.29±12.78%, CD8＋： 37.25±12.30% ）, the percentage of CD3 ＋ T cells in the experimental group increased in the third examination; the percentage of CD4＋ T cells in the experimental group decreased continuously in the second, third, and fourth examinations （50.77±11.05%, 45.40±9.41%, and 41.27±10.62%）, while the percentage of CD8＋ T cells in the experimental group kept increasing （46.07±10.18%, 50.36±10.62%, and 56.40±9.41%） （P〈0.05）. Conclusion The change in T cell subsets precedes that in the blood system in the workers exposed to low levels of benzene.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2012年第10期739-741,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金（30972455）广州市医药卫生科技重点项目（2009-ZDi-18）
关键词
苯
T淋巴细胞
免疫系统
Benzene
T cell subset
immune system
