摘要
目的研究星形胶质细胞中缝隙连接蛋白connexin 43(Cx43)的表达及由其形成的缝隙连接通讯功能的药物调控。方法实验分为正常对照组、全反式维甲酸组(10μmol/L全反式维甲酸作用24 h)及油酸酰胺组(25μmol/L油酸酰胺作用2 h)。western blotting法检测各组星形胶质细胞中Cx43总蛋白的表达;细胞免疫荧光法检测各组星形胶质细胞Cx43胞膜蛋白的表达;荧光示踪法检测各组星形胶质细胞的缝隙连接通讯功能。结果与正常对照组相比,全反式维甲酸能增强星形胶质细胞中Cx43总蛋白的表达(P<0.01),油酸酰胺则能减弱其表达(P<0.01);全反式维甲酸能增强Cx43胞膜蛋白表达,油酸酰胺能减弱其表达;全反式维甲酸能增强星形胶质细胞缝隙连接通讯功能(P<0.01),而油酸酰胺则可显著降低该功能(P<0.01)。结论药物全反式维甲酸及油酸酰胺可以对星形胶质细胞缝隙连接通讯功能进行调控,其机制可能与其影响星形胶质细胞Cx43总蛋白和胞膜蛋白的表达有关。
Objective To determine the expression of connexin 43（Cx43） protein and explore the functional modulation of gap junction intercellular communication in astrocytes.Methods Cultured neonatal SD rat astrocytes were divided into normal control group,all-trans retinoic acid（ATRA） group（treated with 10 μmol/L ATRA for 24 h） and oleamide group（treated with 25 μmol/L oleamide for 2 h）.Western blotting and immunofluorescence assay were used to detect total cellular Cx43 protein expression and Cx43 expression on the surface of the astrocytes,respectively.Parachute assay was used to evaluate the functional changes of gap junction intercellular communication of the astrocytes.Results Compared with the normal control cells,ATRA treatment resulted in a significantly increased expression of total Cx43 protein in the astrocytes（P0.01）,and oleamide significantly suppressed its expression（P0.01）.Similarly,ATRA obviously enhanced while oleamide suppressed Cx43 protein expression on the surface of the astrocytes.The gap junction intercellular communication of the astrocytes was enhanced by ATRA（P0.01） and inhibited by oleamide（P0.01）.Conclusion ATRA and oleamide can modulate gap junction intercellular communication of the astrocytes possibly by regulating the expression of Cx43 protein.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第10期1423-1426,共4页
Journal of Southern Medical University
基金
国家自然科学基金(81001457)
安徽省高等学校省级自然科学研究项目(KJ2012B104)~~
