摘要
目的通过检测献血者血浆中血管性血友病因子(vWF),为其制备冷沉淀和临床应用提供依据。方法应用酶联免疫吸附法检测无偿献血者血浆和普通冰冻血浆中vWF含量。结果无偿献血者血浆中vWF含量为(125.81±48.71)%,去冷沉淀血浆中含量为(25.52±9.29)%。结论无偿献血者血液采集后6h内分离的血浆,其vWF含量稳定,可用于冷沉淀制备和临床应用。
Objective The yon Willebrand Factor （vWF） in the plasma of voluntary blood donors was detected, for the purpose to provide evidence for its clinical application and producing of cryoprecipitate. Methods The concentrations of vWF in fresh plasma and cryoprecipitate reduced plasma were determined by enzyme linked-immuno-sorbent assay. Re- suits The levels of vWF in the fresh and ordinary frozen plasma were （125.81±48.71）% and （25.52±9.29） respec- tively. Conclusion The vWF in the donated blood is stable within 6 hours. The plasma is qualified for vWF supplement and producing cryoprecipitate.
出处
《临床输血与检验》
CAS
2012年第4期297-298,共2页
Journal of Clinical Transfusion and Laboratory Medicine
基金
南京军区南京总医院科研基金(No.2011048)
南京军区医学科技创新重点项目(No.11Z026)资助
关键词
血管性血友病因子
无偿献血者
冷沉淀
普通冰冻血浆
Von Willebrand Factor Voluntary blood donors Cryoprecipitate Ordinary frozen plasma
