摘要
目的探讨Fhit基因在白血病中异常表达的临床意义及其机制。方法收集白血病患者骨髓117例,非白血病患者骨髓28例作为对照组,采用RT-PCR检测Fhit基因mRNA的表达情况、甲基化特异性PCR(MSP)检测Fhit基因启动子区的甲基化情况;探讨临床各因素与Fhit mRNA及基因异常甲基化结果的关系以及Fhit基因甲基化状态与其基因mRNA表达之间的关系。结果 117例白血病患者中,60例(51.28%)不表达Fhit mRNA的白血病骨髓中有45例存在Fhit基因启动子区甲基化,57例(48.72%)白血病骨髓Fhit mRNA阳性表达,仅8例存在甲基化;对照组中Fhit基因mRNA表达27例(96.43%),均未见甲基化现象。Fhit基因在白血病组中mRNA的表达以及甲基化状态与对照组比较差异均有统计学意义(P<0.05)。在临床各参数中,Fhit基因mRNA的表达和甲基化与幼稚细胞多少和肝脾、淋巴结肿大有关,差异均有统计学意义(P<0.05)。结论 Fhit基因的甲基化可能导致其基因的沉默使其mRNA减少或缺失,在白血病的发病机制中起一定作用,Fhit基因甲基化的检测对白血病的诊断和疗效判定可能有一定意义。
Objective To investigate the clinical significance and mechanism of Fhit gene abnormal expres sion in leukemia. Methods Bone marrows from 117 cases with leukemia patients and 28 normal cases（as control） were collected. The level of Fhit transcripts in leukemia patients and control cases were assayed using reverse transcriptase-polymerase chain reaction （RT PCR）,Methylation PCR （MSP） were used to detect the promoter methylation of Fhit gene. The relationship between the expression of Fhit mRNA and abnomal methylation of Fhit gene with clinical data the same as the correlationship between the methyla- tion of Fhit gene with the expression of Fhit mRNA was analyzed. Results In 117 cases, there were 57 cases （48. 72%） expression of Fhit mRNA. In control group, the ratio of Fhit gene expression was 96.43 % （27/28） and no methylation of Fhit gene was found. There was significant difference between the expression of Fhit mRNA in leukemia group and control cases group the same as the methylation preva- lence of Fhit gene （P〈0.05）. Compared the relationship between the expression of Fhit mRNA and abnormal methylation of Fhit gene with clinical data, they were different in immature leukemia cells the same as in liver and spleen or lymph node （P〈0. 05）. Conclusion The abnormal methylation of Fhit gene may be the major mechanism of gene silence in leukemia,resulting to be no expression of Fhit mRNA,and the abnormal methylation of Fhit gene maybe relevant with the process of occurrence and development in the leukemia. There also maybe some effective and significant in the process of diagnosis and curative effect of leukemia patients by detecting the methylation status of Fhit gene.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第10期1246-1249,共4页
Cancer Research on Prevention and Treatment
基金
广西自然科学基金资助项目(2010GXNSFA013184)
广西卫生厅课题(Z2010372)
