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基于MIQE指南的qPCR相对定量法实验教学探讨

Experimental Teaching of Relative Quantification for qPCR Based on MIQE Guideline
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摘要 实时定量qPCR相对定量法是应用最广泛的定量检测基因表达的分子生物学技术,然而长期以来由于缺乏进行qPCR实验的统一标准,从而对实验结果的精确性和文献的可信度产生不利影响。在分析学生应用qPCR研究基因表达的常见问题的基础上,根据qPCR最新国际标准———MIQE指南,探讨qPCR实验教学策略,以规范学生qPCR实验操作,保证实验结果的准确性和可信度。 Relative Quantification for Quantitative Real -Time PCR （qPCR） is the most widely used molecular biology technology for detection of gene expression. However, a lack of a uniform standard for qPCR has long been existed and consequently produces a negative effect on the accuraey of qPCR results and the reliability of publication. Based on the analysis of the problems that students commonly eneounter during the investigation of gene expression using qPCR as well as the novel qPCR international standard ＂ The Minimum Information for Publication of Quantitative Real - Time PCR Experiments＂ （MIQE guideline） , the experimental teaching method of qPCR is discussed to standardize the procedure of qPCR experiment and ensure the aceuraey and reliability of the experiment results.
作者 贺毅 梁丽英 陈晶 陈朝 夏猛 郭超峰 黄琛
机构地区 广西中医学院第一附属医院 广西中医学院基础医学院
出处 《实验室研究与探索》 CAS 北大核心 2012年第9期109-112,117,共5页 Research and Exploration In Laboratory
基金 国家自然科学基金青年基金(81102832) 广西中医学院第一附属医院青年科研基金(GZYQJ2011012)
关键词 MIQE qPCR 基因表达分析 相对定量 实验教学 MIQE qPCR analysis of gene expression relative quantification experimental teaching
分类号 Q819 [生物学—生物工程] G642.0 [文化科学—高等教育学]
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参考文献19

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二级参考文献23

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实验室研究与探索
2012年 第9期

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