摘要
目的:建立苦豆子片的定性定量方法。方法:采用TLC法对方中苦豆子进行定性鉴别,同时采用HPLC法测定苦豆子片中苦参碱的含量。以乙腈-0.01 mol.L-1的碳酸铵溶液(20∶80)为流动相,Phenomenex-Pack Gemini-NX 5U C18110A(4.6mm×250 mm,5μm)色谱柱,检测波长为220 nm,柱温30℃,流速1 mL.min-1。结果:定性鉴别薄层色谱特征明显;HPLC测定苦参碱质量浓度在4.37～26.22 mg.L-1线性关系良好(r=0.999 5),平均回收率102.59%,RSD 0.75%(n=6)。结论:所建标准可用于苦豆子片的质量控制。
Objective： To establish the methods for the quantification and quantitation of Kudouzi tablets. Method： Sophora alopecuroides was identified by TLC. The content of matrine was determined by HPLC. HPLC method was performed on a Phenomenex-Pack Gemini-NX 5U Cls ll0A （4.6 mm x 250 mm, 5 Ixm） column with a mobile phase of acetonitrile-0.01 mol.L-lammonium carbonate water （20： 80）. The flow rate was 1 mL.min-~ The wavelength of detector was 220 nm and the column temperature was set at 30 ~C. Result： Characteristics of identification were very obviously. The linear range of matrine was 4.37-26.22 mg＇L-1 （ r = 0. 999 5） with an average recovery of 102.59% （RSD O. 75% , n =6）. Conclusion： The method can be used for the quality and quantity control of Kudouzi tablets.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期81-83,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新疆"十二五"重大专项"新疆维吾尔药与特色天然药物创制及产业化示范"(201130105)
