摘要
为探明2011年我国猪群仔猪腹泻的病因,采用巢式RT-PCR方法对河南和辽宁省2个规模猪场采集43份出现腹泻的粪便进行猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和轮状病毒(PRoV)的检测,结果表明:43份腹泻样品均为PEDV,没有检测出TGEV和PRoV。对扩增的PEDV进行测序和分析显示,这些PEDV流行毒株均属于基因G2.3亚型,彼此之间的同源性高达99.2%～100%,与2011年韩国和2008年泰国流行的PEDV毒株同属于一个进化分支。将其中3份病料在Vero细胞传代后接种2日龄仔猪,结果发现接种后13～57h内仔猪全部死亡,接种仔猪呈现典型的腹泻症状和病理变化特征,提示了这些PEDV流行毒株具有较强的致病性。
To investigate the occurrence and origin of diarrhea in piglet,porcine epidemic disease virus（PEDV）,transmissible gastroenteritis coronavirus（TGEV） and porcine rotavirus（PRoV） were detected by nested reverse transcription PCR from 43 fecal samples in 2011.All these samples were only positive to PEDV.By comparing the special 412 bp amplifications of M gene with other PEDV reference strains,the epidemic PEDV isolates sharing a highly homology of 99.2%-100% were classified into a new gene subgroup G2.3,which include some epidemic PEDV strains of Korea in 2011 and Thailand in 2008.After 6 series passages in Vero cell,three cultures of PEDV isolates were inoculated into newborn piglets by oral administration.All the piglets displayed diarrhea symptom and died between 13-57 h,which indicated a high pathogenicity.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第10期1423-1428,共6页
Chinese Journal of Veterinary Science
基金
国家支撑计划资助项目(2007BAD86B05)
广西科技攻关课题资助项目(桂科攻0815009-3-9)
关键词
猪流行性腹泻
RT-PCR
检测
致病性
porcine epidemic disease virus
RT-PCR
detection
pathogenicity
