摘要
目的探讨一氧化氮(NO)对疟原虫红内期侵袭相关分子MSP-1、AMA-1和Py 235转录水平影响。方法雌性BALB/c小鼠腹腔注射1×106个致死型约氏疟原虫P.yoelii 17XL感染红细胞,疟原血症达到60%～80%,腹腔注射NO短效发生剂NOC 5,孵育30 min,纯化疟原虫成熟裂殖体,提取总RNA,通过实时定量PCR相对定量方法检测MSP1、AMA1和Py 235转录水平。结果 NO处理后MSP1和AMA1 2种分子mRNA表达水平分别为(0.54±0.02)和(0.37±0.01),与对照组比较,明显降低(P<0.01);作为疟原虫毒力标志的检测基因PYO 5054mRNA表达水平呈下调表现(P<0.05)。结论 NO抑制疟原虫侵袭关键分子转录水平,从而可能影响疟原虫的侵入过程。
Objective To evaluate transcription levels of malaria invasion related molecules of morozolite surface protein-1（MSP-1）,apical membrane antigen-1（AMA-1） and 235 kDa rhoptry protein（Py 235） after short-time nitric oxide（NO） donor（NOC 5） treatment.Methods Female BALB/c mice infected with P.yoelii 17XL were inoculated with NOC 5 by intraperitoneal injection.The mature parasite schizonts was enriched and total RNA was extracted from schizonts.The transcription levels of MSP-1,AMA-1 and Py 235 were detected with real-time PCR.Results The transcription levels of MSP-1 and AMA-1 were down-regulated.As a virulence marker,the gene 5054 of Py 235 showed low transcription level.Conclusion The important invasion related molecules of MSP-1 and AMA-1 were inhibited after NO treatment.The parasites adjusted its transcriptional pattern and adapted to the host immune environment.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2012年第10期1326-1328,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30800962)
关键词
疟疾
NO发生剂
侵袭相关分子
转录
malaria
NO donor
invasion related molecule
transcription
