摘要
目的:观察祛斑汤对雌性C57BL/6J小鼠皮肤黑素合成的影响,为探索该方剂治疗黄褐斑机制提供实验依据。方法:将雌性C57BL/6J小鼠随机分成空白对照组、祛斑汤低(10 g/kg)、中(20 g/kg)、高剂量(40 g/kg)组、维生素C组。各组灌胃给药30 d后皮肤取材。采用免疫组化法观察小鼠皮肤黑素分布。结果:祛斑汤高、中剂量组黑素颗粒阳性反应的黑素细胞平均光密度低于空白对照组,差别有统计学意义(P<0.01)。结论:祛斑汤能抑制雌性C57BL/6J小鼠皮肤中黑素合成、降低黑素分布。
Objective： To observe the effect of Qu-ban Decoction on melanin synthesis in the female C57BL/6J mouse skin and provide the experimental basis for exploring deeply the mechanism of treating melasma by the prescription. Methods： Female C57BL/ 6J0 mice were randomly divided into the blank control group, the Qu-ban Decoction low dose group （ 10 g/kg） , the middle dose group （20 g/kg）, the high dose group （40 g/kg） and the vitamin C group. Take the skin from mice fed with drug for 30 days. Use immu- nohistochemical techniques to observe the melanin distribution and the result was analyzed using the one-way ANOVA statistically. Re- suits： The melanocytes that contained the melanin granules were named positive ceils. The average optical densities of positive cells of the high dose group and the middle dose group were lower than the blank control group. The difference between the high dose group, the middle dose group and the blank control group was significant statistically （P 〈 0.01） . Conclusion： The Qu-ban Decoction can inhibit the melanin synthesis and reduce the melanin distribution in female C57BL/6J mouse skin.
出处
《成都中医药大学学报》
2012年第3期15-18,共4页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省教育厅青年基金(编号:10ZB074)
关键词
祛斑汤
黑素
黄褐斑
Qu-ban Decoction
melanin
melasma
