摘要
建立一种快速、简便,同时针对牛奶中β-内酰胺类抗生素和三聚氰胺残留的检测方法。应用竞争抑制免疫层析原理,研制β-内酰胺类和三聚氰胺胶体金试纸条。该试纸条检测限分别为青霉素G2μg/L、氨苄青霉素3μg/L、阿莫西林3μg/L、苯唑青霉素6μg/L、邻氯青霉素6μg/L、双氯青霉素6μg/L、萘夫西林20μg/L、头孢喹肟20μg/L、头孢哌酮40μg/L、头孢曲松50μg/L、头孢噻呋90μg/L、头孢洛宁10μg/L、三聚氰胺50μg/L,假阳性率低于5%、假阴性率为0,检测时间不超过15min。该方法操作简便、灵敏度高、特异性强,适用于乳品流通环节中β-内酰胺类抗生素和三聚氰胺残留的快速检测。
To establish a rapid and convenient detection method for β-lactam antibiotics and melamine residues in milk. Methods： The colloidal gold strip for β-lactam antibiotics and melamine was developed based on competitive inhibition immunochromatography mechanism. The detection limit of the strip was penicillin G 2 μg/L, ampicillin μg/L, amoxicillin 3 μg/L, oxacillin 6 μg/L, cloxacillin 6 μg/L, dicloxacillin 6 μg/L, nafcillin 20 μg/L, cefquinome 20 μg/L, cefoperazone 40 μg/L, ceftriaxone 50 μg/L, ceftiofur 90 μg/L, cefalonium 10 μg/L, melamine 50 μg/L, respectively. The false positive rate was less than 5%, and the false negative rate was 0. The detection time was less than 15 min. This method is user-friendly with high sensitivity and specificity, and suitable for the rapid detection of β-lactam antibiotics and melamine residues in dairy circulation links.
出处
《食品科技》
CAS
北大核心
2012年第10期267-272,共6页
Food Science and Technology
