摘要
本文报道了同时分析D-木糖、阿拉伯糖、葡萄糖、鼠李糖、D-甘露糖、半乳糖、D-葡萄糖醛酸和D-半乳糖醛酸8种单糖的毛细管电泳紫外检测方法。利用本研究所建立的方法,可以同时检测樟芝多糖水解液中的8种单糖,方法的检测限为0.11～0.22μg/mL,回收率在88%～104%之间,相对标准偏差(RSD)小于3%。本研究为分析表征樟芝多糖提供了一种可靠的方法。
In this study,a method for the simultaneous determination of eight monosaccharides（Xyl,Ara, Glc, Rha, Man, Gal, GlcA, GalA） in the hydrolysate of Antrodia camphorata ＇ s polysaccharide using CE- UV （ ca success pillary electrophoresis with UV detection） was developed. The developed method has been fully applied for the determination of eight monosaccharides in the hydrolysate of Antrodia camphorata ~s polysaccharide with a RSD〈3% ,a recovery of 88%-104% and a detection limit of 0.11 -0.22 μg/mL. This method could be a credible and comparable method for the characterization and quantification of the polysaccharide extracted from Antrodia camphorata ＇s mycelium.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第5期657-660,共4页
Journal of Analytical Science
基金
福建省科技厅重点项目(No.2010H6010
2011Y0016)
关键词
毛细管电泳
樟芝
樟芝多糖
单糖
Capillary electrophoresis
Antrodia camphorata
Antrodia camphorata ＇ s polysaccharide
Monosac-charide
