摘要
目的:选择最适宜的抗原修复法。方法:在免疫组织化学染色中,选用鼠抗人5种单克隆抗体,用不同的抗原修复法,对不同染毒剂量、正常对照暴露大脑皮质及海马,石蜡组织切片,依阳性颗粒表达的情况,确定适宜的抗原修复。结果:抗原修复的方法直接影响到组织中抗原的暴露,高压加热法能把被掩盖或交联变性的抗原暴露或修复,使免疫组化的结果更准确可靠,特别是核阳性组织,适用于高压加热法;电炉煮沸法,阳性结果不佳;酶消化抗原修复法,与高压锅加热抗原修复法的阳性结果无区别。结论:核阳性表达的抗体应选用高压锅抗原修复法,推测核阳性表达的抗体也适用于酶消化抗原修复法。
Objective：To select most appropriate antigen repairing method.Methods：In immunohistochemistry staining,5 monoclonal antibodies of mouse anti-human were selected with different antigen retrieval methods to determine the suitable antigen retrieval according to expressing situation of positive granules for different exposure dose,normal control,brain cortex and hippocampus exposure and paraffin tissue sections.Results：Antigen retrieval method affected directly the exposure of tissue antigen and masked or crosslinking modified antigen can be exposed or repaired by high pressure heating method to make immunohistochemistry results more accurate and reliable,especially nuclear positive tissue that was suitable for high pressure heating method.Positive result was poor with the electric boiling method and there was no difference between the positive results of enzymatic digesting antigen retrieval method and that of highly pressured heating antigen repairing method.Conclusion：Nuclear positive expression antibody should use highly pressured heating antigen repairing method.It is putative that the nuclear positive expression antibody should be also applied to the enzymatic digestion antigen repairing method.
出处
《沈阳医学院学报》
2012年第3期152-153,共2页
Journal of Shenyang Medical College
关键词
免疫组化染色
抗原修复
核阳性表达
immunohistochemistry staining
antigen retrieval
nuclear positive expression
