摘要
对利用聚合酶链式反应 (以下简称 PCR)所克隆的大肠杆菌 bet B基因进行了序列测定 .结果证实获得了该基因的克隆 p XY0 1、p XY0 5以及亚克隆 p XY1 1 .将该基因置于 Ca MV3 5S启动子调控下 ,导入根癌农杆菌 LBA4 40 4中 ,叶盘法转化烟草 ( N icotiana tabaccum) ,在含卡那霉素的选择培养基上筛选抗性芽 ,并进一步诱导小植株的再生 .利用 PCR技术检测基因整合到烟草基因组中 .
The betB gene encoding betaine aldehyde dehydrogenase (BHDH) was amplified by PCR and inserted into the XbaI/KpnI sites of pGEM-3Zf(-) and pGA643. These clones were confirmed by sequencing. The betB gene under the control of CaMV 35S promoter was introduced into Nicotiana tabacum by Agrobacterium tumefaciens mediated procedure. The transformed plantlets of kanamycinresistance were obtained and PCR showed that the betB gene has been integrated into the genome of Nicotiana tabacum.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
2000年第4期414-416,共3页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家自然科学基金资助项目!(39260003)
关键词
大肠杆菌
甜菜碱醛脱氢酶基因
烟草
转化
测序
E.coli
betB gene
Nicotiana tabacum
Agrobacterium tumefaciences
transformation
