摘要
目的建立测定健步关节胶囊中淫羊藿苷含量的高效液相色谱法。方法采用薄层色谱法进行定性鉴别;采用高效液相色谱法对健步关节胶囊中的淫羊藿苷进行定量测定,色谱柱为Zorbax C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(28∶72),检测波长为270 nm,流速为1 mL/min。结果薄层色谱法显示,健步关节胶囊供试品与淫羊藿苷有相同的斑点。淫羊藿苷的线性回归方程为Y=5 E+0.6 X-29 452(r=0.999 6,n=6),进样量线性范围为0.12～0.60μg;平均加收率为99.02%,RSD为1.19%(n=6)。结论该方法简便快捷,结果准确可靠,可作为健步关节胶囊的质量控制方法。
Objective To establish a HPLC method to determine the icariin content in Jianbu Guanjie Capsule. Methods The thin layer chromatography（TLC） was adpoted to conduct the qualitative identification of Jianbu Guanjie Capsule. HPLC was used to quantitatively determine the ieariin content,with Zorbax C18 column （250 mm×4. 6 mm,5 mm） as the chromatography column and acetonitrile-water （28：72） as the mobile phase at the flow rate of 1 mL/min. The detection wave length was 270 nm. Results The TLC chromatogram showed that the test samples of Jianbu Guanjie Capsule and the icarrin standard sample had the same point. HPLC Results showed that the icraiin linear equation was Y = 5E ＋ 0. 6X - 29 452 （ r = 0. 999 6, n = 6）,the linear range was 0. 12 - 0.60 μg. The average recov- ely rate was 99.02%, RSD = 1. 19% （n = 6）. Conclusion This method is simple,convenient,accurate and reliable,and can be used for controlling the quality of Jianbu Guanjie Capsule.
出处
《中国药业》
CAS
2012年第20期42-43,共2页
China Pharmaceuticals
基金
上海市虹口区卫生局医学科研资助重点项目
项目编号:0702-16虹卫[2007]151号
