摘要
目的:探讨抑癌基因WTX在结肠癌组织中的表达水平及其意义。方法:收集经病理学证实的50例结肠癌患者的癌组织及其癌旁组织与远癌组织标本,应用荧光定量PCR(qPCR)及Western blot法检测各组织WTX基因及蛋白的表达水平。结果:相对于远癌组织(1.00±0.00),癌组织与癌旁组织WTX基因表达量分别为0.36±0.17和0.91±0.34,各组织间差异均有统计学意义(均P<0.05)。WTX蛋白的相对表达量在癌组织,癌旁组织,远癌组织分别为0.27±0.11,0.64±0.23,0.72±0.19,各组织间差异均有统计学意义(P<0.05)。结论:抑癌基因WTX的表达降低可能在结肠癌的发生发展进程中起重要作用。
Objective： To investigate the expression level of tumor suppressor gene WTX in colon cancer tissues and its significance. Methods： The specimens of cancerous tissues along with their adjacent and distant tissues from 50 patients with pathologically confirmed colon cancer were collected. The gene and protein expression levels of WTX in each tissue specimen were detected by using Real-time fluorescence quantitative PCR （qPCR） and Western blot analysis, respectivel）n Results： Relative to the value of the distant normal tissues （1.00±0.00）, the WTX gene expression values in the tumor tissues and adjacent tissues were 0.36±0.17 and 0.91 ±0.34 respectively, and there were statistical differences among them （all P〈0.05）. The relative expression levels of WTX protein in the tumor tissues, adjacent and remote tissues were 0.27±0.11, 0.64±0.23 and 0.72±0.19 respectively, and all the differences among them had statistical significance （all P〈0.05）. Conclusion： The decreased expression of tumor suppressor gene WTX may be critically associated with the occurrence and development of colon cancer.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第10期1217-1221,共5页
China Journal of General Surgery
基金
云南省科技厅-昆明医科大学联合专项基金(2010CD165)
云南省应用基础研究重点项目(2011FA029)
