摘要
目的以动脉硬化性闭塞症(ASO)为对象 ,研究白细胞(WBC)活化及粘附状态对血瘀的影响。方法采用(1)硝基蓝四唑(NBT)试验测定WBC的自发活化率(SAR) ;(2)免疫荧光法检测WBC表面粘附分子CD11b/CD18表达 ;(3)剪切力耐受法观测WBC的粘附。结果ASO三种血瘀证WBC的SAR和粘附率显著高于正常(P<0.01) ,粘附分子表达增加 ,全血粘度升高 ,红细胞(RBC)变形性下降。结论血液异常改变是中医血瘀“血行失度”的共同病理基础 ,白细胞高活化状态及粘附性的改变在血细胞微血管嵌塞和组织损伤中起重要作用。因此对血瘀证的治疗应重视抑制WBC的活化和粘附 。
Objective Selecting arteriosclerosis obliterans (ASO) as objects to study the effects of WBC spontaneous activation and adhesion in blood stasis syndrome. Methods (1) Nitro blue tetraxolium test was used to measure the spontaneous activating rate (SAR) of circulating leukocytes. (2) Immunofluorescence test was used to detect the expression of cell adhesion molecule CD11b/CD18 on leukocytes. (3) Shear stress tolerance test was used to observe the adhesion of leukocytes. Results Compared with control group, the SAR and adherent rate of WBC were elevated significantly (P<0.01) with the increased expression of CD11b/CD18, the rise of blood viscosity and the decreased deformability of erythrocyte. Conclusion The abnormal changes of blood is the common pathogenesis of TCM blood stasis. The elevated SAR and adhenion of leukocyte play important roles in microvascular obstruction and tissue injury. So more emphasis should be layed on the inhibition of WBC spontaneous activation、 adhesion、 restraining free radicals and improving the deformability of RBC.
出处
《中国微循环》
2000年第2期116-117,129,共3页
Journal of Chinese Microcirculation
基金
国家中医药管理局科研基金!(95B137)
关键词
血瘀证
白细胞
自发活化
粘附
Blood stasis syndrome White blood cell Spontaneous activation Adhesion
